製品の概要

  • 製品名Anti-c-Fos antibody
    c-Fos 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to c-Fos
  • 特異性Recognizes c-Fos and v-Fos.
  • アプリケーション適用あり: WB, ICC, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Cat, Dog, Pig
  • 免疫原

    Fusion protein:

    W LVQPALVSSV APSQTRAPHP FGVPAPSAGA YSRAGVVKTM TGGRAQSIGR RGKVEQLSPE EEEKRRIRRE RNKMAAAKCR

    , corresponding to N terminal amino acids 75-155 of Human c-Fos.

  • ポジティブ・コントロール
    • HeLa nuclear extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab18119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC
IP
  • 追加情報
    ICC: Use at a concentration of 5 - 10 µg/ml. This concentration has given positive results in HeLa cells that had been serum-starved and then serum-stimulated for 30 minutes prior to fixation with 4% paraformaldehyde followed by 1% Triton-X.
    IP: 4 - 10 µg immunoprecipitates c-Fos from 100µg of a RIPA lysate of human HeLa cells.
    WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 41 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
    • 配列類似性Belongs to the bZIP family. Fos subfamily.
      Contains 1 bZIP domain.
    • 翻訳後修飾Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
      Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Activator protein 1 antibody
      • AP 1 antibody
      • C FOS antibody
      • Cellular oncogene c fos antibody
      • Cellular oncogene fos antibody
      • FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody
      • FBJ murine osteosarcoma viral oncogene homolog antibody
      • FBJ murine osteosarcoma viral v fos oncogene homolog antibody
      • FBJ Osteosarcoma Virus antibody
      • FOS antibody
      • FOS protein antibody
      • FOS_HUMAN antibody
      • G0 G1 switch regulatory protein 7 antibody
      • G0/G1 switch regulatory protein 7 antibody
      • G0S7 antibody
      • Oncogene FOS antibody
      • p55 antibody
      • proto oncogene c Fos antibody
      • Proto oncogene protein c fos antibody
      • Proto-oncogene c-Fos antibody
      • v fos FBJ murine osteosarcoma viral oncogene homolog antibody
      see all

    Anti-c-Fos antibody (ab18119) 使用論文

    ab18119 has not yet been referenced specifically in any publications.

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