The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報 ICC: Use at a concentration of 5 - 10 µg/ml. This concentration has given positive results in HeLa cells that had been serum-starved and then serum-stimulated for 30 minutes prior to fixation with 4% paraformaldehyde followed by 1% Triton-X.
IP: 4 - 10 µg immunoprecipitates c-Fos from 100µg of a RIPA lysate of human HeLa cells.
WB: Use at a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 41 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
配列類似性Belongs to the bZIP family. Fos subfamily. Contains 1 bZIP domain.
翻訳後修飾Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation. Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.