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The antiserum was produced against synthesized phosphopeptide derived from human c-Abl around the phosphorylation site of tyrosine 412 (D-T-YP-T-A). Note: Two forms of c Abl are generated by alternative splicing. Tyr residue at position 412 is present in isoforms 1b of c Abl. The corresponding phosphorylation site in isoforms 1a is at position 393.
Our Abpromise guarantee covers the use of ab47315 in the following tested applications.
|WB||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
ab47315 staining c Abl (phospho Y412) in human platelets by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde and permeabilized with 0.2% Triton x100 before blocking with 10% serum at 200C for 1 hour. Samples were incubated with primary antibody (1/100: in 10% goat serum) for 1 hour 20 minutes at 20°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG used at dilution at 1/400 as secondary antibody.