製品の概要

  • 製品名Anti-c Abl (phospho Y412) antibody
    c Abl 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to c Abl (phospho Y412)
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Abl 1b that contains tyrosine 412. Note: there are two widely expressed forms of c-Abl produced by alternative splicing, designated 1b (the more common form) and 1a. The corresponding phosphorylation site from 1a is tyrosine 393.

  • ポジティブ・コントロール
    • Fibroblasts transfected with oncogenic ∆SH3-Abl.
  • 特記事項


    c-Abl is a 140-150 kDa non-receptor protein tyrosine kinase whose precise functions are not known, but roles for Abl in growth factor and integrin signaling, cell cycle regulation, cytoskeletal reorganization, neurogenesis, and responses to DNA damage and oxidative stress have been suggested. c-Abl kinase activity is increased in vivo by diverse physiological stimuli including ionizing radiation, entry into S phase, integrin activation, and platelet-derived growth factor (PDGF) stimulation. c-Abl contains various protein binding domains that appear to enable it to regulate the functions of many proteins by forming complexes, most notably three isoforms of the oncogenic protein Bcr/Abl. c-Abl becomes fully activated by sequential phosphorylation of tyrosines 412 and 245.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: PBS (without Mg2+ and Ca2+), 1.0mg/ml BSA (IgG, protease free). pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c-Abl. The final product is generated by affinity chromatography using a c-Abl derived peptide that is phosphorylated at tyrosine 245.
  • 一次抗体 備考c-Abl is a 140-150 kDa non-receptor protein tyrosine kinase whose precise functions are not known, but roles for Abl in growth factor and integrin signaling, cell cycle regulation, cytoskeletal reorganization, neurogenesis, and responses to DNA damage and oxidative stress have been suggested. c-Abl kinase activity is increased in vivo by diverse physiological stimuli including ionizing radiation, entry into S phase, integrin activation, and platelet-derived growth factor (PDGF) stimulation. c-Abl contains various protein binding domains that appear to enable it to regulate the functions of many proteins by forming complexes, most notably three isoforms of the oncogenic protein Bcr/Abl. c-Abl becomes fully activated by sequential phosphorylation of tyrosines 412 and 245.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 140 kDa.

ターゲット情報

  • 機能Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
  • 組織特異性Widely expressed.
  • 関連疾患Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻訳後修飾Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
    Isoform IB is myristoylated on Gly-2.
  • 細胞内局在Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Abelson murine leukemia viral oncogene homolog 1 antibody
    • Abelson tyrosine protein kinase 1 antibody
    • Abl 1 antibody
    • ABL antibody
    • ABL proto oncogene 1 non receptor tyrosine kinase antibody
    • ABL1 antibody
    • ABL1_HUMAN antibody
    • bcr/abl antibody
    • bcr/c abl oncogene protein antibody
    • c ABL antibody
    • c abl oncogene 1 non receptor tyrosine kinase antibody
    • c abl oncogene 1 receptor tyrosine kinase antibody
    • c ABL1 antibody
    • JTK7 antibody
    • p150 antibody
    • Proto oncogene tyrosine protein kinase ABL1 antibody
    • Proto-oncogene c-Abl antibody
    • Tyrosine-protein kinase ABL1 antibody
    • v abl Abelson murine leukemia viral oncogene homolog 1 antibody
    • v abl antibody
    see all

Anti-c Abl (phospho Y412) antibody 画像

  • Peptide Competition: Fibroblasts transfected with oncogenic ∆SH3-Abl were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL phospho c-Abl (Tyr 412) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to phospho c-Abl (Tyr 412) blocks the antibody signal, thereby demonstrating the specificity of the antibody.

Anti-c Abl (phospho Y412) antibody (ab4717) 使用論文

This product has been referenced in:
  • Kim SY  et al. Rescue of platinum-damaged oocytes from programmed cell death through inactivation of the p53 family signaling network. Cell Death Differ 20:987-97 (2013). Read more (PubMed: 23598363) »
  • Stevens TL  et al. Using bcr-abl to examine mechanisms by which abl kinase regulates morphogenesis in Drosophila. Mol Biol Cell 19:378-93 (2008). WB . Read more (PubMed: 17959833) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Abl 1b that contains tyrosine 412. The immunogen sequence is approximately 10 amino acid residues. The immunogen wou...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"