製品の概要

  • 製品名Anti-c Abl antibody
    c Abl 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to c Abl
  • アプリケーション適用あり: IHC-P, WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide from the sequence adjacent to protein tyrosine kinase domain of human c-Abl.

  • ポジティブ・コントロール
    • Placenta. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 122.9 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.
  • 組織特異性Widely expressed.
  • 関連疾患Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻訳後修飾Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
    Isoform IB is myristoylated on Gly-2.
  • 細胞内局在Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Abelson murine leukemia viral oncogene homolog 1 antibody
    • Abelson tyrosine protein kinase 1 antibody
    • Abl 1 antibody
    • ABL antibody
    • ABL proto oncogene 1 non receptor tyrosine kinase antibody
    • ABL1 antibody
    • ABL1_HUMAN antibody
    • bcr/abl antibody
    • bcr/c abl oncogene protein antibody
    • c ABL antibody
    • c abl oncogene 1 non receptor tyrosine kinase antibody
    • c abl oncogene 1 receptor tyrosine kinase antibody
    • c ABL1 antibody
    • JTK7 antibody
    • p150 antibody
    • Proto oncogene tyrosine protein kinase ABL1 antibody
    • Proto-oncogene c-Abl antibody
    • Tyrosine-protein kinase ABL1 antibody
    • v abl Abelson murine leukemia viral oncogene homolog 1 antibody
    • v abl antibody
    see all

Anti-c Abl antibody 画像

  • ab15130 staining c-Abl in human placenta by Immunohistochemistry (FFPE-sections)..

  • ab15130 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15130 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10min) Hek293, HepG2, and MCF-7 cells, and also in 100% Methanol fixed (5min) Hela, Hek293, HepG2, and MCF-7 cells.

Anti-c Abl antibody (ab15130) 使用論文

ab15130 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Loading amount 15 µg
Specification MEF
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

投稿 Sep 28 2006

Thank you for this interesting question. The peptide is: ISDEVEKELGK and in the human is located between aa502-512,the kinase domain is located between aa242-aa493. The antibody does not recognize the other Abl family kinase Arg. Please do not hesi...

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