Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
30 µg
Specification
HeLa
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TTBS + 5% BSA
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: 190 kDa
Additional data
Additional Notes
Lane 1: HeLa cells transfected with Empty Vector
Lanes 2 and 3: HeLa cells transfected with vector expressing protein tagged with myc (The untagged version of the protein weight approx. 160 KDa).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 04 2013