Anti-c-Myb 抗体 (ab62824)
Key features and details
- Goat polyclonal to c-Myb
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-c-Myb antibody
c-Myb 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to c-Myb -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee, Zebrafish -
免疫原
Synthetic peptide:
C-QRHYNDEDPEKEKR
, corresponding to internal sequence amino acids 281-294 of Human c-Myb. -
ポジティブ・コントロール
- Recombinant Human c-Myb protein (ab114406) can be used as a positive control in WB. Human Spleen lysate
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62824の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 3 - 6 µg/ml.
|
|
WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
---|
IHC-P
Use a concentration of 3 - 6 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
機能
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
配列類似性
Contains 3 HTH myb-type DNA-binding domains. -
ドメイン
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
翻訳後修飾
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 463016 Chimpanzee
- Entrez Gene: 317776 Cow
- Entrez Gene: 483995 Dog
- Entrez Gene: 4602 Human
- Entrez Gene: 17863 Mouse
- Entrez Gene: 498982 Rat
- Omim: 189990 Human
- SwissProt: P46200 Cow
see all -
別名
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
画像
-
Anti-c-Myb antibody (ab62824) at 0.3 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Detected by chemiluminescence -
ab62824 at 4µg/ml staining c-Myb in paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Inset image is a Negative Control showing paraffin embedded Human Kidney, with no primary antibody.
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (3)
ab62824 は 3 報の論文で使用されています。
- Wang F et al. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. Cell Res 27:416-439 (2017). PubMed: 28244490
- Yi T et al. eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nat Commun 6:7194 (2015). WB . PubMed: 26018492
- Zheng J et al. Activation of hepatic stellate cells is suppressed by microRNA-150. Int J Mol Med 32:17-24 (2013). PubMed: 23604143