Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS
Other product details
Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit FITC
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/200
Additional data
Additional Notes
Asynchronous HeLa were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. Non-specific staining throughout both the nucleus and the cytoplasm was evident and suggests that the antibody does not appear to recognize its expected nuclear epitope under these conditions in these cells. Methanol-fixed samples were also tried and produced 1-2 nuclear foci but exhibited a more significant degree of background cytoplasmic staining.
Abcam response
Thank you for your valuable feedback. ICC/IF was not a tested application for ab77071 and it seems that this antibody does not recognize c-Maf under these experimental conditions. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Mar 29 2010