Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Salivary gland)
Permeabilization
Yes - Triton 0.01%
Specification
Salivary gland
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2000µg/mL · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 0.05% Triton and 2mg/ml BSA
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: anti rabbit Alexa fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Isabelle Miletich
Verified customer
投稿 Apr 19 2017