製品の概要

  • 製品名
    Anti-BZW1 antibody
  • 製品の詳細
    Rabbit polyclonal to BZW1
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    A 16 amino acid peptide from near the C terminal residues of Human BZW1 protein (BAD96965).

  • ポジティブ・コントロール
    • Lymph nodes, HeLa , HepG2 and MCF7 cell lysate

法規制情報

製品の特性

  • 製品の状態
    Lyophilised:Reconstitute in 200ul sterile H2O.
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250 - 1/1000. Predicted molecular weight: 48 kDa.
ELISA 1/20000 - 1/80000.
IHC-P 1/50 - 1/200.

ターゲット情報

  • 機能
    Enhances histone H4 gene transcription but does not seem to bind DNA directly.
  • 配列類似性
    Belongs to the BZW family.
    Contains 1 W2 domain.
  • 発生段階
    Expressed in day 3 embryo.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Basic leucine zipper and W2 domain containing protein 1 antibody
    • Basic leucine zipper and W2 domain-containing protein 1 antibody
    • basic leucine-zipper protein BZAP45 antibody
    • BZAP45 antibody
    • bzw1 antibody
    • BZW1_HUMAN antibody
    • KIAA0005 antibody
    • Nbla10236 antibody
    • Protein Orf antibody
    • putative protein product of Nbla10236 antibody
    see all

Anti-BZW1 antibody 画像

  • All lanes : Anti-BZW1 antibody (ab85090) at 1/500 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate


    Predicted band size : 48 kDa
    Observed band size : 48 kDa
  • Immunohistochemical analysis of BZW1 expression in paraffin embedded formalin fixed lymph node section using 1/100 ab85090. note cytoplasmic staining.

Anti-BZW1 antibody (ab85090) 使用論文

ab85090 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunoprecipitation
Immuno-precipitation step
Other - Dynabead IgG
Sample
Human Tissue lysate - whole (brain)
Specification
brain
Total protein in input
50 µg
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Abcam user community

Verified customer

投稿 Dec 06 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Cell (neuronal)
Specification
neuronal
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C
Type
Indirect
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Abcam user community

Verified customer

投稿 Sep 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuronal)
Loading amount
75 µg
Specification
neuronal
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
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Abcam user community

Verified customer

投稿 Sep 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (neuronal)
Loading amount
75 µg
Specification
neuronal
Gel Running Conditions
Non-reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Sep 03 2012

Thank you for your reply.   The antibody, ab85090 has the possibility to recognize BZW2, but the possibility is low. The antigen peptide is 15aa peptide and 9aa are the same in BZW2.   Please let me know if there is anything else I can help you with.

Thank you for your reply. I am contacting the lab to see what the exact immunogen sequence ab85090 is. When I find out I will compare that to the BZW2 sequence and see what the level of homology is. If there is anything else I can do in...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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