製品の概要

  • 製品名Anti-BubR1 antibody
    BubR1 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to BubR1
  • 特異性ab28193 recognises Bub1b protein
  • アプリケーション適用あり: IP, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Recombinant fragment, corresponding to amino acids 232-603 of Mouse BubR1

  • ポジティブ・コントロール
    • Mitotic NIH/3T3 cells

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab28193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 120 kDa).
IHC-P Use at an assay dependent dilution. PubMed: 20807801
ICC/IF Use at an assay dependent concentration. PubMed: 22024163

ターゲット情報

  • 機能Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.
  • 組織特異性Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.
  • 関連疾患Note=Defects in BUB1B are associated with tumor formation.
    Defects in BUB1B are the cause of premature chromatid separation trait (PCS) [MIM:176430]. PCS consists of separate and splayed chromatids with discernible centromeres and involves all or most chromosomes of a metaphase. It is found in up to 2% of metaphases in cultured lymphocytes from approximately 40% of normal individuals. When PCS is present in 5% or more of cells, it is known as the heterozygous PCS trait and has no obvious phenotypic effect, although some have reported decreased fertility. Inheritance is autosomal dominant.
    Defects in BUB1B are the cause of mosaic variegated aneuploidy syndrome (MVA) [MIM:257300]. MVA is a severe autosomal recessive developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. The proportion of aneuploid cells varies but is usually more than 25% and is substantially greater than in normal individuals. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases. MVA is caused by biallelic mutations in the BUB1B gene.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.
    Contains 1 BUB1 N-terminal domain.
    Contains 1 protein kinase domain.
  • ドメインThe D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase.
    The BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.
  • 翻訳後修飾Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.
    Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.
    Ubiquitinated. Degradated by the proteasome.
    Sumoylated by SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.
    Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.
  • 細胞内局在Cytoplasm. Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta homolg of S. cerevisiae BUB 1 antibody
    • Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody
    • BUB 1B antibody
    • BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody
    • Bub1A antibody
    • BUB1B antibody
    • BUB1B_HUMAN antibody
    • BUB1beta antibody
    • BUBR1 antibody
    • Budding Uninhibited by Benzimidazoles 1 beta antibody
    • Budding uninhibited by benzimidazoles 1 homolog beta (yeast) antibody
    • hBUBR1 antibody
    • MAD3/BUB1 related protein kinase antibody
    • MAD3/BUB1-related protein kinase antibody
    • MAD3L antibody
    • Mitotic checkpoint gene BUB1B antibody
    • Mitotic checkpoint kinase MAD3L antibody
    • Mitotic checkpoint serine/threonine protein kinase BUB1 beta antibody
    • Mitotic checkpoint serine/threonine-protein kinase BUB1 beta antibody
    • MVA1 antibody
    • OTTHUMP00000160319 antibody
    • Protein SSK1 antibody
    • SSK 1 antibody
    • SSK1 antibody
    see all

Anti-BubR1 antibody 画像

  • ab28193 staining BubR1 in Mouse metaphase I oocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with Triton X-100. Samples were incubated with primary antibody (1/200) for 15 hours at 4°C. A FITC-conjugated Donkey anti-sheep IgG polyclonal (1/500) was used as the secondary antibody. The signal of BuRI was detected near the chromosome kinetocore of meiosis I stage oocyte.

    See Abreview

  • Anti-BubR1 antibody (ab28193) at 1/3000 dilution + Mouse MII oocytes whole cell lysate at 60 cells

    Secondary
    Rabbit Anti-Sheep IgG H&L (HRP) (ab6747) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 120 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    This image is courtesy of an Abreview submitted by Naoko Kimura

    See Abreview

  • Mouse embryos were treated briefly with acidic Tyrode’s solution to remove their zona pellucida. They were fixed in 4% paraformaldehyde in PBS and permeabilized in 1% Triton X-100 in PBS. Unspecific binding was blocked with 0.5% Tween-20 in PBS containing 5% bovine serum albumin. Subsequently, the embryos were incubated overnight with ab28193 at a 1/200 dilution. Unspecific binding was blocked with 50% normal goat serum, followed by incubation with an Alexa-Fluor 488 conjugated secondary antibody at a 1/500 dilution.

Anti-BubR1 antibody (ab28193) 使用論文

This product has been referenced in:
  • Huang CJ  et al. The SUMO Protease SENP3 Orchestrates G2-M Transition and Spindle Assembly in Mouse Oocytes. Sci Rep 5:15600 (2015). Mouse . Read more (PubMed: 26493771) »
  • Petsalaki E  et al. Phosphorylation at serine 331 is required for Aurora B activation. J Cell Biol 195:449-66 (2011). WB, ICC/IF . Read more (PubMed: 22024163) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample Human Cell (PC3)
Specification PC3
Permeabilization Yes - 0.5% Triton-X100 10 mins
Fixative Formaldehyde
Username

Dr. Ruth Thompson

Verified customer

投稿 Sep 12 2014

Application Immunoprecipitation
Immuno-precipitation step Other - Dynabeads
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Total protein in input 1000 µg
Username

Dr. Ruth Thompson

Verified customer

投稿 Sep 12 2014

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing
Sample Hamster Cell lysate - whole cell (Chinese Hamster Ovary cells (TREx CHO cells))
Specification Chinese Hamster Ovary cells (TREx CHO cells)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Eleni Petsalaki

Verified customer

投稿 Dec 10 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Sample Hamster Cell (Chinese Hamster Ovary cells (TREx CHO cells))
Specification Chinese Hamster Ovary cells (TREx CHO cells)
Permeabilization Yes - 0.5% Triton
Fixative Paraformaldehyde
Username

Dr. Eleni Petsalaki

Verified customer

投稿 Dec 10 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Metaphase I oocyte)
Specification Metaphase I oocyte
Permeabilization Yes - Triton- X100
Fixative Paraformaldehyde
Username

直子 木村

Verified customer

投稿 Oct 09 2013

Application Western blot
Loading amount 60 cells
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (MII oocytes)
Specification MII oocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

直子 木村

Verified customer

投稿 Oct 02 2013

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Colon carcinoma)
Specification Colon carcinoma
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton in PBS
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
Username

Dr. Eleni Petsalaki

Verified customer

投稿 Dec 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (embryonic)
Specification embryonic
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Mar 07 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"