製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Bub3
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 275 and 325 of Human Bub3 (Swiss-Prot O43684).

  • ポジティブ・コントロール
    • Human metastatic lymph node. Human prostate adenocarcinoma.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab84448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
    • 配列類似性
      Belongs to the WD repeat BUB3 family.
      Contains 5 WD repeats.
    • 細胞内局在
      Nucleus. Chromosome > centromere > kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Aa2-050 antibody
      • AU019800 antibody
      • AU021329 antibody
      • AU043350 antibody
      • AW146323 antibody
      • BUB 3 antibody
      • BUB 3L antibody
      • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog antibody
      • bub3 antibody
      • BUB3 budding uninhibited by benzimidazoles 3 homolog antibody
      • BUB3 mitotic checkpoint protein antibody
      • BUB3_HUMAN antibody
      • BUB3L antibody
      • Budding uninhibited by benomyl antibody
      • Budding uninhibited by benzimidazoles 3 antibody
      • Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) antibody
      • Budding uninhibited by benzimidazoles 3 homolog (yeast) antibody
      • Budding uninhibited by benzimidazoles 3 homolog antibody
      • Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody
      • C78067 antibody
      • hBUB3 antibody
      • Mitotic checkpoint component antibody
      • Mitotic checkpoint protein bub3 antibody
      • OTTHUMP00000020679 antibody
      • OTTHUMP00000020680 antibody
      • WD repeat type I transmembrane protein A72.5 antibody
      see all

    画像

    • ab84448, at 1/250 dilution, staining Bub3 in formalin-fixed, paraffin-embedded human metastatic lymph node tissue by Immunohistochemistry using DAB detection.
    • ab84448, at 1/250 dilution, staining Bub3 in formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue by Immunohistochemistry using DAB detection.

    プロトコール

    参考文献

    ab84448 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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