製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Bub3
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, EMSAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide derived from residues 300 to the C-terminus of Human Bub3.

  • ポジティブ・コントロール
    • HeLa Nuclear Extract;

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).
EMSA Use at an assay dependent concentration. PubMed: 22210889

ターゲット情報

  • 機能
    Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
  • 配列類似性
    Belongs to the WD repeat BUB3 family.
    Contains 5 WD repeats.
  • 細胞内局在
    Nucleus. Chromosome > centromere > kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Aa2-050 antibody
    • AU019800 antibody
    • AU021329 antibody
    • AU043350 antibody
    • AW146323 antibody
    • BUB 3 antibody
    • BUB 3L antibody
    • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog antibody
    • bub3 antibody
    • BUB3 budding uninhibited by benzimidazoles 3 homolog antibody
    • BUB3 mitotic checkpoint protein antibody
    • BUB3_HUMAN antibody
    • BUB3L antibody
    • Budding uninhibited by benomyl antibody
    • Budding uninhibited by benzimidazoles 3 antibody
    • Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) antibody
    • Budding uninhibited by benzimidazoles 3 homolog (yeast) antibody
    • Budding uninhibited by benzimidazoles 3 homolog antibody
    • Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody
    • C78067 antibody
    • hBUB3 antibody
    • Mitotic checkpoint component antibody
    • Mitotic checkpoint protein bub3 antibody
    • OTTHUMP00000020679 antibody
    • OTTHUMP00000020680 antibody
    • WD repeat type I transmembrane protein A72.5 antibody
    see all

画像

  • Anti-Bub3 antibody (ab4180) at 1/500 dilution + HeLa Nuclear Extract at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

    Predicted band size: 37 kDa
    Observed band size: 38 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 minute


    Western blot of ab4180 on 20ug HeLa Nuclear Extract at 1/500.

    Secondary ab: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000 - 1 min exposure)

プロトコール

参考文献

This product has been referenced in:
  • Zhu S  et al. A motif from Lys216 to Lys222 in human BUB3 protein is a nuclear localization signal and critical for BUB3 function in mitotic checkpoint. J Biol Chem 290:11282-92 (2015). Read more (PubMed: 25814666) »
  • Burns SS  et al. Histone deacetylase inhibitor AR-42 differentially affects cell-cycle transit in meningeal and meningioma cells, potently inhibiting NF2-deficient meningioma growth. Cancer Res 73:792-803 (2013). WB . Read more (PubMed: 23151902) »

See all 5 Publications for this product

レビューと Q&A

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa Cell)
Permeabilization
Yes - 0.5% Trition-X
Specification
HeLa Cell
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Hazheen Shirnekhi

Verified customer

投稿 Mar 01 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (293)
Loading amount
10 µg
Specification
293
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 31 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
10 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

投稿 Mar 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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