製品の概要

  • 製品名Anti-Bub3 antibody
    Bub3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bub3
  • アプリケーション適用あり: WB, EMSAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Xenopus laevis, Zebrafish
  • 免疫原

    Synthetic peptide derived from residues 300 to the C-terminus of Human Bub3.

  • ポジティブ・コントロール
    • HeLa Nuclear Extract;

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS. pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4180 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).
EMSA Use at an assay dependent concentration. PubMed: 22210889

ターゲット情報

  • 機能Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
  • 配列類似性Belongs to the WD repeat BUB3 family.
    Contains 5 WD repeats.
  • 細胞内局在Nucleus. Chromosome > centromere > kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Aa2-050 antibody
    • AU019800 antibody
    • AU021329 antibody
    • AU043350 antibody
    • AW146323 antibody
    • BUB 3 antibody
    • BUB 3L antibody
    • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog antibody
    • bub3 antibody
    • BUB3 budding uninhibited by benzimidazoles 3 homolog antibody
    • BUB3 mitotic checkpoint protein antibody
    • BUB3_HUMAN antibody
    • BUB3L antibody
    • Budding uninhibited by benomyl antibody
    • Budding uninhibited by benzimidazoles 3 antibody
    • Budding uninhibited by benzimidazoles 3 homolog (S. cerevisiae) antibody
    • Budding uninhibited by benzimidazoles 3 homolog (yeast) antibody
    • Budding uninhibited by benzimidazoles 3 homolog antibody
    • Budding uninhibited by benzimidazoles 3, S. cerevisiae, homolog of antibody
    • C78067 antibody
    • hBUB3 antibody
    • Mitotic checkpoint component antibody
    • Mitotic checkpoint protein bub3 antibody
    • OTTHUMP00000020679 antibody
    • OTTHUMP00000020680 antibody
    • WD repeat type I transmembrane protein A72.5 antibody
    see all

Anti-Bub3 antibody 画像

  • Anti-Bub3 antibody (ab4180) at 1/500 dilution + HeLa Nuclear Extract at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

    Predicted band size : 37 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

Anti-Bub3 antibody (ab4180) 使用論文

This product has been referenced in:
  • Burns SS  et al. Histone deacetylase inhibitor AR-42 differentially affects cell-cycle transit in meningeal and meningioma cells, potently inhibiting NF2-deficient meningioma growth. Cancer Res 73:792-803 (2013). WB . Read more (PubMed: 23151902) »
  • Bava FA  et al. CPEB1 coordinates alternative 3'-UTR formation with translational regulation. Nature 495:121-5 (2013). Read more (PubMed: 23434754) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293)
Loading amount 10 µg
Specification 293
Gel Running Conditions Reduced Denaturing (12.5%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Oct 31 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 10 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

投稿 Mar 18 2009

Thank you for your enquiry. Having performed homology searches of the immunogens of our Bub3 antibodies with the Drosophila protein I regret to inform you that neither antibody seem suitable for this species due to low sequence homology. I'm sorr...

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Thank you for your enquiry. This should clear things up for you - ab4180 is the antibody (Rabbit polyclonal to Bub3) and ab13739 is the blocking peptide that can be used with ab4180. If you have any additional questions, please contact us again.

This antibody has been tested briefly in Immunofluorescence in U20S cells (human). IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"