製品の概要

  • 製品名Anti-Bub1 antibody [2096C1a]
    Bub1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2096C1a] to Bub1
  • アプリケーション適用あり: Dot blot, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to Human Bub1 (internal sequence).
    Database link: O43683

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)ab51269 was purified from culture supernatant of the hybridoma, cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • ポリ/モノモノクローナル
  • クローン名2096C1a
  • アイソタイプIgG1
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab51269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 120 kDa.

ターゲット情報

  • 機能Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
  • 組織特異性High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.
    Contains 1 BUB1 N-terminal domain.
    Contains 1 protein kinase domain.
  • ドメインThe KEN box is required for its ubiquitination and degradation.
    BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR. Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction.
    Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.
  • 細胞内局在Nucleus. Chromosome > centromere > kinetochore. Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, CASC5 and INCENP are required for kinetochore localization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Bub1 antibody
    • BUB1 budding uninhibited by benzimidazoles 1 homolog antibody
    • BUB1 budding uninhibited by benzimidazoles 1 homolog (yeast) antibody
    • BUB1 mitotic checkpoint serine/threonine kinase antibody
    • BUB1, S. cerevisiae, homolog of antibody
    • BUB1_HUMAN antibody
    • BUB1A antibody
    • BUB1L antibody
    • Budding uninhibited by benzimidazoles 1 (yeast homolog) antibody
    • Budding uninhibited by benzimidazoles 1 homolog antibody
    • Budding uninhibited by benzimidazoles 1, S. cerevisiae, homolog of antibody
    • hBUB1 antibody
    • Homolog of mitotic checkpoint gene BUB1 antibody
    • Mitotic checkpoint gene BUB1 antibody
    • Mitotic checkpoint serine/threonine protein kinase BUB1 antibody
    • Mitotic checkpoint serine/threonine-protein kinase BUB1 antibody
    • Mitotic spindle checkpoint kinase antibody
    • Putative serine/threonine protein kinase antibody
    see all

Anti-Bub1 antibody [2096C1a] 画像



  • Predicted band size : 120 kDa

    Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: Bub1 knockout HAP1 cell lysate (40 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: F9 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab51269 observed at 125 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab51269 was shown to recognize Bub1 when Bub1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Bub1 knockout samples were subjected to SDS-PAGE. Ab51269 and ab181602 (loading control to GAPDH) were diluted at 1 µg/ml  and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with IRDye® 800CW Goat anti-Mouse IgG (H + L) and IRDye® 680 Goat anti-Rabbit IgG (H + L) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

  • Anti-Bub1 antibody [2096C1a] (ab51269)

    Predicted band size : 120 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Western blot analysis of immunised recombinant protein

Anti-Bub1 antibody [2096C1a] (ab51269) 使用論文

ab51269 has not yet been referenced specifically in any publications.

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