Anti-Bu1a + Bu1b 抗体 [AV20] (Biotin) (ab25166)

製品の概要

  • 製品名Anti-Bu1a + Bu1b antibody [AV20] (Biotin)
    Bu1a + Bu1b 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AV20] to Bu1a + Bu1b (Biotin)
  • 標識Biotin
  • 特異性This antibody recognizes a monomorphic determinant on the Bu1 B cell associated alloantigens of both RPL 6 (Bu1a) and 7 (Bu1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds.
  • アプリケーション適用あり: Flow Cyt, IP, IHC-Frmore details
  • 種交差性
    交差種: Chicken
    非交差種: Turkey
  • 免疫原

    Tissue/ cell preparation: Bursal cells from one day old H.B15 (Bu1a/b ) strain chickens.

  • 特記事項


製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノモノクローナル
  • クローン名AV20
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Acetone fixed.

ターゲット情報

  • 関連性The Bu1 [chB6] molecule is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has long been used as an allotypic marker in important studies of B cell development, though its function is unknown. It has no recognizable similarity to known mammalian molecules and thus represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. The sequences of the different alleles of this gene revealed a higher level of polymorphism than expected. A restriction fragment length polymorphism linked to the CHB6 gene has been used to determine its location on the linkage map of the chicken genome, which will allow the definitive evaluation of reported associations with disease resistance. Bu1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
  • 細胞内局在Type I membrane protein.
  • 参照データベース
  • 別名
    • B6.1 antibody
    • B6.2 antibody
    • Bu 1a antibody
    • Bu 1b antibody
    • LOC396098 antibody
    see all

Anti-Bu1a + Bu1b antibody [AV20] (Biotin) (ab25166) 使用論文

ab25166 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"