製品の概要

  • 製品名Anti-Bu1a + Bu1b antibody [AV20]
    Bu1a + Bu1b 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AV20] to Bu1a + Bu1b
  • アプリケーション適用あり: IP, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Chicken
    非交差種: Turkey
  • 免疫原

    Tissue/ cell preparation: Bursal cells from one day old H.B15 (Bu1a/b) strain chickens.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名AV20
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25400 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. Fix with acetone
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性The Bu1 [chB6] molecule is expressed on chicken B cells throughout most of their development, as well as on some non lymphoid cells. It has long been used as an allotypic marker in important studies of B cell development, though its function is unknown. It has no recognizable similarity to known mammalian molecules and thus represents a unique B cell marker. Its presence in chickens may be related to differences in the properties of B cell development between chickens and mammalian species. The sequences of the different alleles of this gene revealed a higher level of polymorphism than expected. A restriction fragment length polymorphism linked to the CHB6 gene has been used to determine its location on the linkage map of the chicken genome, which will allow the definitive evaluation of reported associations with disease resistance. Bu1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.
  • 細胞内局在Type I membrane protein.
  • 参照データベース
  • 別名
    • B6.1 antibody
    • B6.2 antibody
    • Bu 1a antibody
    • Bu 1b antibody
    • LOC396098 antibody
    see all

Anti-Bu1a + Bu1b antibody [AV20] (ab25400) 使用論文

This product has been referenced in:
  • Bradaric MJ  et al. Immune Cells in the Normal Ovary and Spontaneous Ovarian Tumors in the Laying Hen (Gallus domesticus) Model of Human Ovarian Cancer. PLoS One 8:e74147 (2013). IHC-P, Flow Cyt ; Chicken . Read more (PubMed: 24040191) »
  • Rothwell CJ  et al. Identification of chicken Bu-1 alloantigens using the monoclonal antibody AV20. Vet Immunol Immunopathol 55:225-34 (1996). Read more (PubMed: 9014319) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"