Anti-BTK (phospho Y223) 抗体 [EP420Y] (ab68217)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP420Y] to BTK (phospho Y223)
- Suitable for: WB, IP, Dot blot
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-BTK (phospho Y223) antibody [EP420Y]
BTK 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP420Y] to BTK (phospho Y223) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, Dot blotmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab214589) -
ポジティブ・コントロール
- WB: K562 and Ramos cell lysates treated with pervanadate. IP: Ramos cell lysates treated with pervanadate. Dot Blot: BTK (phospho Y223) phospho peptide.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP420Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68217の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with BTK peptide (ab214589).
For unpurified, use 1/10000 - 1/50000. |
|
IP |
1/20.
For unpurified, use 1/40. |
|
Dot blot |
Use at an assay dependent concentration.
|
特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).Can be blocked with BTK peptide (ab214589). For unpurified, use 1/10000 - 1/50000. |
IP
1/20. For unpurified, use 1/40. |
Dot blot
Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes. -
関連疾患
Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD). -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
翻訳後修飾
Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein. -
細胞内局在
Cytoplasm. Membrane. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 695 Human
- Entrez Gene: 12229 Mouse
- Entrez Gene: 367901 Rat
- Omim: 300300 Human
- SwissProt: Q06187 Human
- SwissProt: P35991 Mouse
- Unigene: 159494 Human
- Unigene: 4475 Mouse
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別名
- Agammaglobulinaemia tyrosine kinase antibody
- AGMX 1 antibody
- AGMX1 antibody
see all
画像
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All lanes : Anti-BTK (phospho Y223) antibody [EP420Y] (ab68217) at 1/1000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate
Lane 3 : Ramos treated with 1mM pervanadate for 30 min whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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ab68217 (purified) at 1/50 immunoprecipitating BTK (phospho Y223) in Ramos cells (Lane 1) treated with 1 mM Pervanadate for 30 min. For western blotting, a HRP-conjugated anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Dot blot analysis of BTK (phospho Y223) phospho peptide (Lane 1) and BTK phospho peptide (Lane 2) labeling BTK (phospho Y223) with ab68217 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-BTK (phospho Y223) antibody [EP420Y] (ab68217) at 1/10000 dilution (purified)
Lane 1 : Untreated K562 cell lysate
Lane 2 : K562 cells treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab68217 は 19 報の論文で使用されています。
- Chen T et al. The effect of geniposide on chronic unpredictable mild stress-induced depressive mice through BTK/TLR4/NF-?B and BDNF/TrkB signaling pathways. Phytother Res 35:932-945 (2021). PubMed: 33164233
- Eisenmann ED et al. Intentional Modulation of Ibrutinib Pharmacokinetics through CYP3A Inhibition. Cancer Res Commun 1:79-89 (2021). PubMed: 34950932
- Sheng L et al. Overexpression of Fc?RIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. Int J Mol Med 46:1409-1422 (2020). PubMed: 32945349
- Liu S et al. The protective effect of Geniposide on diabetic cognitive impairment through BTK/TLR4/NF-?B pathway. Psychopharmacology (Berl) 237:465-477 (2020). PubMed: 31811349
- Kim E et al. The BET inhibitor GS-5829 targets chronic lymphocytic leukemia cells and their supportive microenvironment. Leukemia 34:1588-1598 (2020). PubMed: 31862959