Anti-BTK (phospho Y223) 抗体 (ab51210)

製品の概要

  • 製品名Anti-BTK (phospho Y223) antibody
    BTK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BTK (phospho Y223)
  • 特異性ab51210 detects endogenous levels of BTK only when phosphorylated at tyrosine 222.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthesized phosphopeptide derived from human BTK around the phosphorylation site of tyrosine 223 (A-L-YP-D-Y).

  • ポジティブ・コントロール
    • Extracts from Hela cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab51210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/40000.
WB 1/300 - 1/1000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).

ターゲット情報

  • 機能Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
  • 関連疾患Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
    Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • 翻訳後修飾Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
  • 細胞内局在Cytoplasm. Membrane. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Agammaglobulinaemia tyrosine kinase antibody
    • AGMX 1 antibody
    • AGMX1 antibody
    • AT antibody
    • ATK antibody
    • B cell progenitor kinase antibody
    • B-cell progenitor kinase antibody
    • BPK antibody
    • Bruton agammaglobulinemia tyrosine kinase antibody
    • Bruton tyrosine kinase antibody
    • Bruton’s Tyrosine Kinase antibody
    • Btk antibody
    • BTK_HUMAN antibody
    • dominant-negative kinase-deficient Brutons tyrosine kinase antibody
    • IMD 1 antibody
    • IMD1 antibody
    • MGC126261 antibody
    • MGC126262 antibody
    • OTTHUMP00000063593 antibody
    • PSCTK 1 antibody
    • PSCTK1 antibody
    • truncated Bruton agammaglobulinemia tyrosine kinase antibody
    • Tyrosine protein kinase BTK antibody
    • Tyrosine-protein kinase BTK antibody
    • tyrosine-protein kinase BTK isoform (lacking exon 14 antibody
    • XLA antibody
    see all

Anti-BTK (phospho Y223) antibody 画像

  • All lanes : Anti-BTK (phospho Y223) antibody (ab51210)

    Lane 1 : Extracts from Hela cells treated with serum (10%, 15min) with no immunizing peptide
    Lane 2 : Extracts from Hela cells treated with serum (10%, 15min) with immunizing peptide


    Predicted band size : 76 kDa

Anti-BTK (phospho Y223) antibody (ab51210) 使用論文

ab51210 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

We have some conjugation kits which may be of use to you in biotinylating the anti-BTK (phospho Y223) antibody (ab51210). More information on these kits can be found from the following links:

http://www.abcam...

Read More

Thank you for your reply.

I would be pleased to provide you with the Abtrial testing discount codes. In order to do so, could you please confirm which distributor you will be purchasing the antibodies from?

I look forward to receiving...

Read More

Thank you for contacting us and your interest in our products.

The antibodies you have chosen for your ELISA should work well together. The immunogen used to raise the mouse monoclonal ab54219 does not cover the Y223 region of BTK and they sh...

Read More

Following up my message from yesterday, the laboratory tells me that they will not be able to provide this Anti-BTK (phospho Y223) antibody without glycerol. Please let me know if you have any questions or if we can help in any other way.

Thank you for your enquiry. I have checked these lot numbers and found that they come from the same master stock reference 5119, they were just aliquoted at different times and should therefore behave in an identical manner. Could the small change...

Read More

Thank you for your enquiry. I am sorry to hear that you have been experiencing problems with ab51210 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event t...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"