製品の概要

  • 製品名
    Anti-BTG1 antibody [2095C1a]
    BTG1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2095C1a] to BTG1
  • 特異性
    ab50991 has not yet been tested on endogenous protein in cell lysate.
  • アプリケーション
    適用あり: Dot blot, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment of BTG1 (Human)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 19 kDa.

ターゲット情報

  • 関連性
    BTG1 belongs to the BTG family of proteins.FUNCTION: Anti-proliferative protein. Its expression is associated with the early G1 phase of the cell cycle. A chromosomal aberration involving BTG1 may be a cause of a form of B cell chronic lymphocytic leukemia; translocation t(8;12)(q24;q22) with MYC.
  • 参照データベース
  • 別名
    • B cell translocation gene 1 protein antibody
    • B cell translocation gene 1, anti proliferative antibody
    • BTG1 protein antibody
    • Protein BTG1 antibody

画像

プロトコール

参考文献

This product has been referenced in:
  • Liu C  et al. BTG1 potentiates apoptosis and suppresses proliferation in renal cell carcinoma by interacting with PRMT1. Oncol Lett 10:619-624 (2015). WB, IHC . Read more (PubMed: 26622543) »

See 1 Publication for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (RS411 (B-cell leukemia))
Loading amount
100000 cells
Specification
RS411 (B-cell leukemia)
Treatment
5µM MG132 over night
Gel Running Conditions
Reduced Denaturing
Blocking step
2% milk + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Dec 01 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - whole cell (RS411 (B-cell leukemia))
Specification
RS411 (B-cell leukemia)
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde in EDTA/EGTA/H
Detection step
Real-time PCR
Positive control
ChIP on TBP and Glucocorticoid Receptor
Negative control
Isotype control
Username

Abcam user community

Verified customer

投稿 Dec 01 2010

Thank you for your patience in this matter. The originator of ab50991 (ab50991 BTG1 antibody [2095C1a]) has provided the following information: The WB image was obtained under the following conditions: -Primary Ab dilution; 1:100 -Secondary Ab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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