製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BTF3
  • アプリケーション
    適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Bat
  • 免疫原

    Synthetic peptide corresponding to a region between amino acids 35-70 of Human BTF3 (NP_001032726.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 10 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      General transcription factor. BTF3 can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
    • 配列類似性
      Belongs to the NAC-beta family.
      Contains 1 NAC-A/B (NAC-alpha/beta) domain.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Basic transcription factor 3 antibody
      • BETA NAC antibody
      • BTF 3 antibody
      • btf3 antibody
      • BTF3_HUMAN antibody
      • BTF3a antibody
      • BTF3b antibody
      • NACB antibody
      • Nascent polypeptide associated complex beta polypeptide antibody
      • RNA polymerase B transcription factor 3 antibody
      • Transcription factor BTF3 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and human prostate carcinoma (right) tissues labelling BTF3 with ab113974 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of BTF3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab113974 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-BTF3 antibody which recognizes a upstream epitope was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

      Predicted band size : 22 kDa.

    参考文献

    ab113974 has not yet been referenced specifically in any publications.

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab113974.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録