Detection of BTBD15 by Western Blot of Immunprecipitate.
An antibody to BTBD15 at 1µg/ml staining BTBD15 in Jurkat whole cell lysate following immunoprecipitation.
Lane 1) IP using ab117814 at 6µg/mg lysate
Lane 2) IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised chemiluminescence with a 10 second exposure.