The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 87 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 20 µg/ml.
Required for the polarization of forebrain neurons which endows axons and dendrites with distinct properties, possibly by locally regulating phosphorylation of microtubule-associated proteins (By similarity). May be involved in the regulation of G2/M arrest in response to UV- or methyl methane sulfonate (MMS)-induced, but not IR-induced, DNA damage. Phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo.
Widely expressed, with highest levels in brain and testis. Protein levels remain constant throughout the cell cycle.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily. Contains 1 protein kinase domain. Contains 1 UBA domain.
Cytoplasm. Nucleus. Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage.
ab115475, at 5µg/ml, staining BRSK1 in formalin-fixed, paraffin-embedded Human brain cortex tissue by immunohistochemistry using a biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-BRSK1 antibody (ab115475)
Lane 1 : Anti-BRSK1 antibody (ab115475) at 0.5 µg/ml Lane 2 : Anti-BRSK1 antibody (ab115475) at 1 µg/ml
Lane 1 : Human brain tissue lysate Lane 2 : Human brain tissue lysate