製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BRP44
  • 特異性
    ab111380 detects endogenous levels of total BRP44 protein.
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal region of Human BRP44.

  • ポジティブ・コントロール
    • Human lung carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 0.88% Sodium chloride
    Note: without Mg2+ and Ca2+
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab111380 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab111380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

Anti-BRP44 antibody 画像

  • ab111380 at 1/50 dilution staining BRP44 in paraffin-embedded Human Lung carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

プロトコール

Anti-BRP44 antibody (ab111380) 使用論文

ab111380 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Leukemia cell lines)
Gel Running Conditions
Reduced Denaturing (4-20% and 18%)
Loading amount
40 µg
Specification
Leukemia cell lines
Blocking step
Licor:PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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投稿 Nov 04 2016

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (12)
Sample
Human Cell lysate - whole cell (Lane 1 U2OS, Lane 2 MIN6)
Specification
Lane 1 U2OS, Lane 2 MIN6
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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