Anti-BROX 抗体 (ab122174)
Key features and details
- Rabbit polyclonal to BROX
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-BROX antibody -
製品の詳細
Rabbit polyclonal to BROX -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: LGNLVKNTLE KCQRENGFIY FQKIPTEAPQ LELKANYGLV EPIPFEFPPT SVQWTPETLA AFDLTKRPKD DSTKPKPEEE VKPVKEPDIK PQKDTGCYIS, corresponding to amino acids of Human BROX
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ポジティブ・コントロール
- ICC/IF: U-2 OS cells. IHC-P: Human urinary bladder tissue. WB: RT4 and U-251 MG cell lysate. Human liver and tonsil tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122174の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/250 - 1/500.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
特記事項 |
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IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/250 - 1/500. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
ターゲット情報
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配列類似性
Belongs to the BROX family.
Contains 1 BRO1 domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 148362 Human
- SwissProt: Q5VW32 Human
- Unigene: 552608 Human
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別名
- BRO1 domain and CAAX motif containing antibody
- BRO1 domain containing protein antibody
- BRO1 domain- and CAAX motif-containing protein antibody
see all
画像
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Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus. Recommended concentration of ab122174 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab122174, at 1/500 dilution, staining BROX in paraffin-embedded human urinary bladder tissue by Immunohistochemistry.
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All lanes : Anti-BROX antibody (ab122174) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab122174 は論文での使用が確認できていません。