Anti-BRN4 抗体 (ab104562)
Key features and details
- Rabbit polyclonal to BRN4
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-BRN4 antibody
BRN4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BRN4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat
交差が予測される動物種: Mouse, Rabbit, Horse, Cow, Human, Pig, Macaque monkey, Chinese hamster -
免疫原
Synthetic peptide corresponding to Rat BRN4 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab124139) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following tissue lysayes: Rat Brain; Rat Hippocampus; Rat Cerebellum.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104562の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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IHC-P |
Use a concentration of 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Probable transcription factor which exert its primary action widely during early neural development and in a very limited set of neurons in the mature brain. -
組織特異性
Brain specific. -
関連疾患
Defects in POU3F4 are a cause of deafness X-linked type 2 (DFNX2) [MIM:304400]. A mixed type of deafness characterized by both conductive hearing loss resulting from stapes (perilymphatic gusher) fixation and progressive sensorineural deafness. -
配列類似性
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5456 Human
- Entrez Gene: 18994 Mouse
- Entrez Gene: 29589 Rat
- Omim: 300039 Human
- SwissProt: P49335 Human
- SwissProt: P62515 Mouse
- SwissProt: P62516 Rat
- Unigene: 2229 Human
see all -
別名
- Brain specific homeobox POU domain protein 4 antibody
- Brain-4 antibody
- Brain-specific homeobox/POU domain protein 4 antibody
see all
画像
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All lanes : Anti-BRN4 antibody (ab104562) at 1 µg/ml
Lane 1 : Rat Hippocampus Tissue Lysate
Lane 2 : Cerebellum Rat Tissue Lysate
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Additional bands at: 190 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes -
IHC image of BRN4 staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab104562, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab104562 は 1 報の論文で使用されています。
- Masawa M et al. REST Inactivation and Coexpression of ASCL1 and POU3F4 Are Necessary for the Complete Transformation of RB1/TP53-Inactivated Lung Adenocarcinoma into Neuroendocrine Carcinoma. Am J Pathol 192:847-861 (2022). PubMed: 35367201