製品の概要

  • 製品名Anti-BRN3B / POU4F2 antibody
    BRN3B / POU4F2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BRN3B / POU4F2
  • 特異性This antibody reacts with BRN3B / POU4F2.
  • アプリケーション適用あり: ICC/IF, ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human BRN3B/ POU4F2 aa 1-50 (N terminal).
    Sequence:

    MMMMSLNSKQAFSMPHGGSLHVEPKYSALHSTSPGSSAPIAPSASSPSSS

  • ポジティブ・コントロール
    • WB: HepG2 cell lysate. IF/ICC: SKNSH cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

WB Use a concentration of 0.2 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
  • 組織特異性Brain. Seems to be specific to the retina.
  • 配列類似性Belongs to the POU transcription factor family. Class-4 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 細胞内局在Nucleus speckle.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Brain specific homeobox/POU domain protein 3B antibody
    • Brain-3B antibody
    • Brain-specific homeobox/POU domain protein 3B antibody
    • Brn-3B antibody
    • BRN3.2 antibody
    • BRN3B antibody
    • Brn3b POU domain transcription factor antibody
    • class 4 antibody
    • PO4F2_HUMAN antibody
    • POU class 4 homeobox 2 antibody
    • POU domain antibody
    • POU domain class 4 transcription factor 2 antibody
    • POU domain protein antibody
    • POU4F2 antibody
    • transcription factor 2 antibody
    see all

Anti-BRN3B / POU4F2 antibody 画像

  • ICC/IF image of ab56026 stained Malme-3M cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56026, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-BRN3B / POU4F2 antibody (ab56026) at 0.2 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

Anti-BRN3B / POU4F2 antibody (ab56026) 使用論文

This product has been referenced in:
  • Tucker BA  et al. Use of a synthetic xeno-free culture substrate for induced pluripotent stem cell induction and retinal differentiation. Stem Cells Transl Med 2:16-24 (2013). Read more (PubMed: 23283489) »

See 1 Publication for this product

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Thank you very much for your interest our products.



To our knowledge, we do not have an alternative antibody which has been tested in IHC so far, although we have two mor...

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Thank you for your telephone enquiry yesterday afternoon.

I am sorry to confirm that ab32264 BRN3B / POU4F2 antibodyis not longer available.

As discussed on the telephone, we do have an alternative, ab56026. I can recommend to rev...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"