製品の概要

  • 製品名Anti-BRN3A antibody [EP1972Y]
    BRN3A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1972Y] to BRN3A
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cyt, IP, IHC - Wholemountmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from the C terminal region of Human BRN3A

  • ポジティブ・コントロール
    • WB: Human brain lysate; IHC: Human placenta tissue; ICC/IF: HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
IHC - Wholemount Use at an assay dependent concentration.

ターゲット情報

  • 機能Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
  • 組織特異性Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye.
  • 配列類似性Belongs to the POU transcription factor family. Class-4 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 発生段階Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Brain specific homeobox/POU domain protein 3A antibody
    • Brain-3A antibody
    • Brain-specific homeobox/POU domain protein 3A antibody
    • Brn 3.0 antibody
    • BRN 3A antibody
    • Brn-3A antibody
    • Brn3 antibody
    • BRN3A antibody
    • class 4 antibody
    • FLJ13449 antibody
    • Homeobox/POU domain protein RDC 1 antibody
    • Homeobox/POU domain protein RDC-1 antibody
    • Homeobox/POU domain protein RDC1 antibody
    • Oct T1 antibody
    • Oct-T1 antibody
    • OctT1 antibody
    • PO4F1_HUMAN antibody
    • POU class 4 homeobox 1 antibody
    • POU domain antibody
    • POU Domain Class 4 Transcription Factor 1 antibody
    • Pou4f1 antibody
    • RDC 1 antibody
    • RDC1 antibody
    • transcription factor 1 antibody
    see all

Anti-BRN3A antibody [EP1972Y] 画像

  • All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213)

    Lane 1 : LNCaP
    Lane 2 : SW480
    Lane 3 : PC-3
    Lane 4 : Human brain lysate


    Predicted band size : 43 kDa
    Observed band size : 43 kDa
  • Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using 1/100 ab81213.
  • Immunofluorescent staining of HeLa cells using 1/100 ab81213.
  • All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) at 1/2000 dilution

    Lane 1 : Adult mouse cerebellum lysate
    Lane 2 : Adult mouse lower brainstem lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated Mouse anti-Rabbit IgG monoclonal. at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa


    Exposure time : 2 minutes

    This image is courtesy of an Anonymous Abreview.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab81213 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81213, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-BRN3A antibody [EP1972Y] (ab81213) 使用論文

This product has been referenced in:
  • Lee KS  et al. Human sensory neurons derived from induced pluripotent stem cells support varicella-zoster virus infection. PLoS One 7:e53010 (2012). Flow Cyt ; Human . Read more (PubMed: 23285249) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (mouse eye)
Permeabilization Yes - 0.3% triton X-100
Specification mouse eye
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 18 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Retinal ganglion cells)
Permeabilization Yes - Triton-X-100
Specification Retinal ganglion cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Dr. David L Rousso

Verified customer

投稿 Aug 11 2015

With the frozen sections, did you do a fix and permeabilize step. I didnt notice that in your protocol. If you haven't then that 's probably the reason for no staining. Its important to do that so that the epitope is accessible.
I would also just ...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
Specification Dorsal Root Ganglia (DRG) neurons
Fixative Paraformaldehyde
Permeabilization Yes - tween 20
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
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Abcam user community

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投稿 Oct 18 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application IHC - Wholemount
Sample Mouse Tissue (Brain explant)
Specification Brain explant
Username

Abcam user community

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投稿 Oct 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (cerebellum & lower portion of brainstem (adult))
Loading amount 20 µg
Specification cerebellum & lower portion of brainstem (adult)
Gel Running Conditions Reduced Denaturing (12 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Feb 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"