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RabMAb

Anti-BRN3A 抗体 [EP1972Y] (ab81213)

製品の概要

  • 製品名
    Anti-BRN3A antibody [EP1972Y]
    BRN3A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1972Y] to BRN3A
  • アプリケーション
    適用あり: WB, Flow Cytmore details
    適用なし: ICC/IF or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from the C terminal region of Human BRN3A

  • ポジティブ・コントロール
    • WB: Human brain lysate; ICC/IF: HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81213 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 43 kDa.

For unpurified use at 1/500 - 1/1000. 

Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/90. 

  • 追加情報
    Is unsuitable for ICC/IF or IP.
  • ターゲット情報

    • 機能
      Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
    • 組織特異性
      Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye.
    • 配列類似性
      Belongs to the POU transcription factor family. Class-4 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • 発生段階
      Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Brain specific homeobox/POU domain protein 3A antibody
      • Brain-3A antibody
      • Brain-specific homeobox/POU domain protein 3A antibody
      • Brn 3.0 antibody
      • BRN 3A antibody
      • Brn-3A antibody
      • Brn3 antibody
      • BRN3A antibody
      • class 4 antibody
      • FLJ13449 antibody
      • Homeobox/POU domain protein RDC 1 antibody
      • Homeobox/POU domain protein RDC-1 antibody
      • Homeobox/POU domain protein RDC1 antibody
      • Oct T1 antibody
      • Oct-T1 antibody
      • OctT1 antibody
      • PO4F1_HUMAN antibody
      • POU class 4 homeobox 1 antibody
      • POU domain antibody
      • POU Domain Class 4 Transcription Factor 1 antibody
      • Pou4f1 antibody
      • RDC 1 antibody
      • RDC1 antibody
      • transcription factor 1 antibody
      see all

    画像

    • Immunofluorescent staining of HeLa cells using 1/100 unpurified ab81213.

    • All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) (unpurified)

      Lane 1 : LNCaP
      Lane 2 : SW480
      Lane 3 : PC-3
      Lane 4 : Human brain lysate


      Predicted band size : 43 kDa
      Observed band size : 43 kDa
    • All lanes : Anti-BRN3A antibody [EP1972Y] (ab81213) at 1/2000 dilution (unpurified)

      Lane 1 : Adult mouse cerebellum lysate
      Lane 2 : Adult mouse lower brainstem lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      HRP-conjugated Mouse anti-Rabbit IgG monoclonal. at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 43 kDa
      Observed band size : 43 kDa


      Exposure time : 2 minutes

      This image is courtesy of an Anonymous Abreview.

      See Abreview

    • Overlay histogram showing SH-SY5Y cells stained with unpurified ab81213 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81213, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    参考文献

    This product has been referenced in:
    • Çerman E  et al. Retinal Electrophysiological Effects of Intravitreal Bone Marrow Derived Mesenchymal Stem Cells in Streptozotocin Induced Diabetic Rats. PLoS One 11:e0156495 (2016). IF . Read more (PubMed: 27300133) »
    • Lee KS  et al. Human sensory neurons derived from induced pluripotent stem cells support varicella-zoster virus infection. PLoS One 7:e53010 (2012). Flow Cyt ; Human . Read more (PubMed: 23285249) »

    See all 2 Publications for this product

    レビューと Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (mouse eye)
    Permeabilization
    Yes - 0.3% triton X-100
    Specification
    mouse eye
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Mar 18 2016

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Retinal ganglion cells)
    Permeabilization
    Yes - Triton-X-100
    Specification
    Retinal ganglion cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Kris Pike

    Verified customer

    投稿 Aug 11 2015

    With the frozen sections, did you do a fix and permeabilize step. I didnt notice that in your protocol. If you haven't then that 's probably the reason for no staining. Its important to do that so that the epitope is accessible.
    I would also just ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
    Specification
    Dorsal Root Ganglia (DRG) neurons
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - tween 20
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 18 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Brain explant)
    Specification
    Brain explant
    Username

    Abcam user community

    Verified customer

    投稿 Oct 05 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (cerebellum & lower portion of brainstem (adult))
    Loading amount
    20 µg
    Specification
    cerebellum & lower portion of brainstem (adult)
    Gel Running Conditions
    Reduced Denaturing (12 %)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Feb 15 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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