製品の概要

  • 製品名Anti-BRN3A antibody
    BRN3A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BRN3A
  • 特異性Immunostaining for Brn3a protein with ab30880 was observed in dorsal root ganglion neurons.
  • アプリケーション適用あり: IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide derived from within residues 400 to the C-terminus of Mouse BRN3A.

    (Peptide available as ab30867.)

  • ポジティブ・コントロール
    • rat brain, rat dorsal root ganglion

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab30880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 0.54 µg/ml.

ターゲット情報

  • 機能Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
  • 組織特異性Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye.
  • 配列類似性Belongs to the POU transcription factor family. Class-4 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 発生段階Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Brain specific homeobox/POU domain protein 3A antibody
    • Brain-3A antibody
    • Brain-specific homeobox/POU domain protein 3A antibody
    • Brn 3.0 antibody
    • BRN 3A antibody
    • Brn-3A antibody
    • Brn3 antibody
    • BRN3A antibody
    • class 4 antibody
    • FLJ13449 antibody
    • Homeobox/POU domain protein RDC 1 antibody
    • Homeobox/POU domain protein RDC-1 antibody
    • Homeobox/POU domain protein RDC1 antibody
    • Oct T1 antibody
    • Oct-T1 antibody
    • OctT1 antibody
    • PO4F1_HUMAN antibody
    • POU class 4 homeobox 1 antibody
    • POU domain antibody
    • POU Domain Class 4 Transcription Factor 1 antibody
    • Pou4f1 antibody
    • RDC 1 antibody
    • RDC1 antibody
    • transcription factor 1 antibody
    see all

Anti-BRN3A antibody 画像

  • Immuofluorescent staining for BRN3A in the rat dorsal root ganglion using Rabbit polyclonal to BRN3A (ab30880; images taken with X20 objective). ab30880 was used at 1/1000 (0.54µg/ml) incubated overnight at room temperature. Secondary antibody used was anti-rabbit Alexa Fluor 488 at 1/1000 incubated for 2 hours at room temperature. Rat tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.

Anti-BRN3A antibody (ab30880) 使用論文

This product has been referenced in:
  • Mollema NJ  et al. Nuclear Receptor Rev-erb Alpha (Nr1d1) Functions in Concert with Nr2e3 to Regulate Transcriptional Networks in the Retina. PLoS One 6:e17494 (2011). IHC-P ; Mouse . Read more (PubMed: 21408158) »
  • Yamada M  et al. Origin of climbing fiber neurons and their developmental dependence on Ptf1a. J Neurosci 27:10924-34 (2007). IHC ; Mouse . Read more (PubMed: 17928434) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (Ms Kidney lysate)
Loading amount 30 µg
Specification Ms Kidney lysate
Gel Running Conditions Reduced Denaturing (10% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Jul 16 2008

I'm sorry to hear you are experiencing problems with ab30880. This antibody has not been tested in IHC on formalin fixed paraffin sections, but in perfusion fixed frozen sections as detailed in the legend of the image on the datasheet. I am therefore ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"