製品の概要

  • 製品名Anti-BRMS1 antibody
    BRMS1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to BRMS1
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-247 of Human BRMS1

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57082 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 28 kDa.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
  • 組織特異性Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.
  • 配列類似性Belongs to the BRMS1 family.
  • 細胞内局在Nucleus. Cytoplasm. Predominantly nuclear.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AV003220 antibody
    • AW554636 antibody
    • Breast cancer metastasis suppressor 1 antibody
    • Breast cancer metastasis-suppressor 1 antibody
    • BRMS1 antibody
    • BRMS1_HUMAN antibody
    • DKFZp564A063 antibody
    • MGC95128 antibody
    see all

Anti-BRMS1 antibody 画像

  • Anti-BRMS1 antibody (ab57082) + Human liver

    Predicted band size : 28 kDa
    Observed band size : 28 kDa
  • Sandwich ELISA. The detection limit for recombinant tagged BRMS1 is approximately 3ng/ml as a capture antibody.
  • Anti-BRMS1 antibody (ab57082) + immunogen

    Predicted band size : 28 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab57082 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57082, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-BRMS1 antibody (ab57082) 使用論文

This product has been referenced in:
  • Al-Alwan M  et al. Fascin is a key regulator of breast cancer invasion that acts via the modification of metastasis-associated molecules. PLoS One 6:e27339 (2011). WB ; Human . Read more (PubMed: 22076152) »

See 1 Publication for this product

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