製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BRIT1
  • アプリケーション
    適用あり: IP, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen, corresponding to amino acids 555-701 of Human BRIT1 (Q8NEM0).

  • ポジティブ・コントロール
    • Human cerebral cortex RT-4 cell lysate U-251 MG cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab121277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Implicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex.
  • 組織特異性
    Expressed in fetal brain, liver and kidney.
  • 関連疾患
    Defects in MCPH1 are the cause of microcephaly primary type 1 (MCPH1) [MIM:251200]; also known as true microcephaly or microcephaly vera. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits. This entity is inherited as autosomal recessive trait.
  • 配列類似性
    Contains 3 BRCT domains.
  • 細胞内局在
    Cytoplasm > cytoskeleton > centrosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BRCT repeat inhibitor of TERT expression 1 antibody
    • BRIT 1 antibody
    • FLJ12847 antibody
    • Hypothetical protein FLJ12847 antibody
    • MCPH 1 antibody
    • MCPH1 antibody
    • MCPH1_HUMAN antibody
    • MCT antibody
    • Microcephalin 1 antibody
    • Microcephalin antibody
    • Microcephaly primary autosomal recessive 1 antibody
    see all

画像

  • All lanes : Anti-BRIT1 antibody (ab121277) at 1/250 dilution

    Lane 1 : Markers (kDa)
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human plasma
    Lane 5 : Liver tissue lysate
    Lane 6 : Tonsil tissue lysate

    Secondary
    HPR conjugated anti-rabbit antibody at 1/3000 dilution
    Developed using the ECL technique
  • ab121277, at 1/75, staining BRIT1 in neuronal cells of paraffin embedded Human cerebral cortex by Immunohistochemistry.
  • Immunohistochemical staining of Human testis shows strong positivity in a subset of cells in seminiferus ducts using ab121277

参考文献

This product has been referenced in:
  • Ge C  et al. The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex. PLoS One 10:e0144957 (2015). Read more (PubMed: 26683461) »
  • Ge C  et al. BRUCE regulates DNA double-strand break response by promoting USP8 deubiquitination of BRIT1. Proc Natl Acad Sci U S A 112:E1210-9 (2015). Read more (PubMed: 25733871) »

See all 2 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (U2OS and RPE)
Total protein in input
3.5 µg
Specification
U2OS and RPE
Immuno-precipitation step
Protein A
Username

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投稿 Apr 16 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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