Synthetic peptide conjugated to KLH derived from within residues 1400 - 1500 of Human BRG1.
(Peptide available as ab13736.)
Our Abpromise guarantee covers the use of ab4081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP | Use at an assay dependent concentration. PubMed: 20522713 | |
WB | 1/500 - 1/1000. Detects a band of approximately 235 kDa (predicted molecular weight: 185 kDa). |
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: BRG1 knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (40 µg)
Lane 4: K562 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab4081 observed at 240 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab4081 was shown to recognize BRG1 when BRG1 knockout samples were used, along with additional cross-reactive bands. Wild-type and BRG1 knockout samples were subjected to SDS-PAGE. Ab4081 and ab18058 (loading control to Vinculin) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) with Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
Rabbit polyclonal to BRG1 (ab4081) at 1/500 on HeLa Nuclear extract (20 ug per lane).
Lane 1: ab4081 (1/500)
Lane 2: ab4081 (1/500) + blocking peptide (1 ug)
Secondary antibody: Goat anti-rabbit (HRP) - ab6721
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"