製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to BRF2
  • アプリケーション
    適用あり: ChIP, IP, ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide: QAARQAATSVPNPP, corresponding to C terminal amino acids 406-419 of Human BRF2.

  • ポジティブ・コントロール
    • HeLa cell lysate. Human placenta lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab17011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 20418882
ELISA 1/16000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human BRF2 peptide (ab23297).

ターゲット情報

  • 機能
    General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.
  • 配列類似性
    Belongs to the TFIIB family.
    Contains 1 TFIIB-type zinc finger.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B-related factor 2 antibody
    • BRF-2 antibody
    • BRF2 antibody
    • BRF2_HUMAN antibody
    • BRFU antibody
    • FLJ11052 antibody
    • hBRFU antibody
    • hTFIIIB50 antibody
    • RNA polymerase III transcription initiation factor BRF2 antibody
    • TFIIIB50 antibody
    • Transcription factor IIB- related factor TFIIIB50 antibody
    • Transcription factor IIIB 50 kDa subunit antibody
    see all

Anti-BRF2 antibody 画像



  • Predicted band size : 48 kDa


    ab17011 staining (1.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab17011 used in ChIP. For details please see Abreview.

Anti-BRF2 antibody (ab17011) 使用論文

This product has been referenced in:
  • Lockwood WW  et al. Integrative genomic analyses identify BRF2 as a novel lineage-specific oncogene in lung squamous cell carcinoma. PLoS Med 7:e1000315 (2010). WB ; Human . Read more (PubMed: 20668658) »
  • Oler AJ  et al. Human RNA polymerase III transcriptomes and relationships to Pol II promoter chromatin and enhancer-binding factors. Nat Struct Mol Biol 17:620-8 (2010). IP ; Human . Read more (PubMed: 20418882) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ChIP
Sample
Human Cell lysate - nuclear (HeLa S3)
Specification
HeLa S3
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde, 1%
Detection step
Real-time PCR
Positive control
Human U6 locus, chr15:65919436-65919686 (hg18)
Negative control
Gene desert locus, chr10:43939489-43939659 (hg18)
Username

Dr. Andrew Oler

Verified customer

投稿 Apr 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録