製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BRF1
  • 特異性
    This antibody recognizes BRF1
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Cow, Dog, Saccharomyces cerevisiae, Drosophila melanogaster
  • 免疫原

    Synthetic peptide: PALGAEPIKP SAVLVESGPV SYHPEEDADE EDAEDEDGEP CVSALQMMGG, corresponding to a region within C terminal amino acids 612-662 of Mouse BRF1

  • ポジティブ・コントロール
    • NIH3T3 lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay was 1:62500.

WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 74 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
  • 配列類似性
    Belongs to the TFIIB family.
    Contains 1 TFIIB-type zinc finger.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B related factor 1 antibody
    • B-related factor 1 antibody
    • BRF antibody
    • BRF-1 antibody
    • brf1 antibody
    • BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) antibody
    • General transcription factor IIIB, 90kD subunit antibody
    • GTF3B antibody
    • hBRF antibody
    • hTFIIIB90 antibody
    • RNA polymerase III antibody
    • subunit 2 antibody
    • TAF3B2 antibody
    • TAF3C antibody
    • TAFIII90 antibody
    • TATA box binding protein (TBP) associated factor 3C antibody
    • TATA box binding protein (TBP) associated factor, RNA polymerase III, GTF3B subunit 2 antibody
    • TATA box-binding protein-associated factor antibody
    • TBP associated factor, RNA polymerase III, 90kD antibody
    • TF3B_HUMAN antibody
    • TF3B90 antibody
    • TFIIIB90 antibody
    • Transcription factor IIIB 90 kDa subunit antibody
    • Transcription initiation factor IIIB antibody
    see all

画像

  • Anti-BRF1 antibody (ab49122) at 1.25 µg/ml + NIH3T3 lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 74 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab49122 has not yet been referenced specifically in any publications.

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