製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Brevican
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH conjugated synthetic peptide corresponding to a region within C terminal amino acids 722-749 of Human Brevican (NP_068767.3).

  • ポジティブ・コントロール
    • CEM cell line lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab107197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 99 kDa.

ターゲット情報

  • 関連性
    Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C-terminal epitope EAMESE.
  • 細胞内局在
    Isoform 1 is secreted, extracellular space, extracellular matrix; Isoform 2 is found on the membrane; Lipid-anchor, GPI-anchor.
  • 参照データベース
  • 別名
    • BCAN antibody
    • BEHAB antibody
    • Brain enriched hyaluronan binding protein antibody
    • Brevican core protein antibody
    • Brevican core protein isoform 1 antibody
    • Brevican core protein isoform 2 antibody
    • Brevican proteoglycan antibody
    • Chondroitin sulfate proteoglycan 7 antibody
    • Chondroitin sulfate proteoglycan BEHAB antibody
    • CSPG7 antibody
    • MGC13038 antibody
    see all

画像

  • Anti-Brevican antibody (ab107197) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size : 99 kDa

プロトコール

参考文献

ab107197 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Ictidomys tridecemlineatus Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
50 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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投稿 Dec 09 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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