BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
Key features and details
- Sensitivity: 40 cells/well
- Sample type: Adherent cells, Suspension cells
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
製品の概要
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製品名
BrdU Cell Proliferation ELISA Kit (colorimetric)
BrdU キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Sandwich (qualitative) -
検出感度
< 40 cells/well -
全工程の試験時間
2h 30m -
ステップ
Multiple steps standard assay -
製品の概要
BrDU Cell proliferation ELISA kit (ab126556) is an indirect ELISA kit for the detection of BrdU incorporation into newly synthesized DNA of actively proliferating cells. It involves incorporation of BrdU into cells cultured in microtiter plates using the cell layer as the solid phase. During the final 2 to 24 hours of culture BrdU is added to wells of the microtiter plate. BrdU will be incorporated into the DNA of dividing cells. To enable antibody binding to the incorporated BrdU cells must be fixed, permeabilized and the DNA denatured. This is all done in one step by treatment with Fixing Solution. Detector anti-BrdU monoclonal antibody is pipetted into the wells and allowed to incubate for one hour, during which time it binds to any incorporated BrdU. Unbound antibody is washed away and horseradish peroxidase-conjugated goat anti-mouse antibody is added, which binds to the Detector Antibody.
The horseradish peroxidase catalyzes the conversion of the chromogenic substrate tetra-methylbenzidine (TMB) from a colorless solution to a blue solution (or yellow after the addition of stopping reagent), the intensity of which is proportional to the amount of incorporated BrdU in the cells. The colored reaction product is quantified using a spectrophotometer.
The resultant assay is sensitive, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture.
Store kit at +4°C immediately upon receipt except for the Prediluted Anti-BrdU Detector Antibody and Peroxidase Goat anti-mouse IgG (2000X) which must be stored at -20°C.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 200 tests 500X BrdU Reagent 1 x 15µl Conjugate Diluent 1 x 25ml Fixing Solution 2 x 20ml Peroxidase Goat anti-mouse IgG (2000X) 1 x 15µl Plate wash concentrate (50X) 1 x 90ml Prediluted anti-BrdU detecting antibody 1 x 20ml Stop Solution 1 x 25ml TMB Substrate 1 x 25ml -
研究分野
関連製品
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画像
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Cell proliferation measured in fibroblasts showing cell amounts vs. optical densities
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Detection of RH7777 (adherent) cells per well with 2 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal -to-noise ratio.
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Detection of Jurkat cells (non-adherent) per well with 24 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.
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Detection of MCF7 cells per well with 2 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.
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Detection of MCF7 cells per well with 24 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (160)
ab126556 は 160 報の論文で使用されています。
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