製品の概要

  • 製品名Anti-BrdU antibody [MoBu-1]
    BrdU 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [MoBu-1] to BrdU
  • 特異性This antibody reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunofluorescence staining. The reaction shows a clear, nuclear confined speckled pattern. It reacts also specifically with 5-bromouridine (BrU).
  • アプリケーション適用あり: ICC, IHC-P, ICC/IF, Flow Cytmore details
  • 免疫原

    Chemical/ Small Molecule corresponding to BrdU.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use a concentration of 2 µg/ml.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 2 µg/ml.
Flow Cyt Use a concentration of 1 - 2 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 細胞内局在Nuclear
  • 別名
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody [MoBu-1] 画像

  • ICC/IF image of ab8039 stained HeLa cells, both BrdU treated (left image) and normal cells (right image). The cells were 100% methanol fixed (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were  then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8039, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Positive staining can be seen in the BrdU treated cells, but not in the normal cells, demonstrating specificity for BrdU.

  • IHC image of ab8039 staining, both in normal and BrdU treated rat liver formalin fixed paraffin embedded tissue sections, performed on a Leica Bond

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Flow cytometry analysis of CEM (human acute lymphoblastic leukemia) cells labelling BrdU with ab8039 at 1 µg/mL. Goat anti-mouse IgG was used as the secondary antibody. The individual cell cycle phases (S, G1, G2/M-phase) are indicated on the figure. 

Anti-BrdU antibody [MoBu-1] (ab8039) 使用論文

This product has been referenced in:
  • Köpper F  et al. Damage-induced DNA replication stalling relies on MAPK-activated protein kinase 2 activity. Proc Natl Acad Sci U S A 110:16856-61 (2013). Read more (PubMed: 24082115) »
  • Gonzalez-Nunez V  et al. Characterization of drCol 15a1b: a novel component of the stem cell niche in the zebrafish retina. Stem Cells 28:1399-411 (2010). IHC-P . Read more (PubMed: 20549708) »

See all 5 Publications for this product

Product Wall

Thank you for your inquiry and for rating your experience with us.

I just wanted to follow up on your query with some additional information.

For ab8039, this antibody can be used for detection of BrdU and BrU. We have not tested it...

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Thank you for contacting us. We have not tested these antibodies for any cross-reactivity with IdU. Please let me know if you have any further questions.

Thank you for your reply.

We know of one publication at this moment in time where this antibody was in flow cytometry (also stated in the reference tab of the datasheet):

http://www.ncbi.nlm.nih.gov/pubmed/18625847?dopt=Abstract Read More

Thank you for your inquiry.

I can confirm that ab8039 reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle.

It does not matter what species the DNA is from. If BrdU is incorporated into human or ovine D...

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Yes. Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10-3 N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"