製品の概要

  • 製品名
    Anti-BrdU antibody [IIB5]
    BrdU 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [IIB5] to BrdU
  • 特異性
    This antibody also reacts with bromodeoxyuridine when incorporated into nuclear DNA. It cross reacts with iododeoxyuridine (IdU).
  • アプリケーション
    適用あり: ICC/IF, Flow Cyt, IHC-Fr, IHC-Pmore details
  • 免疫原

    Chemical/ Small Molecule conjugated to BSA.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab8955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.

Using immunocytochemistry, a combination of this antibody and Mouse monoclonal to Mitotic Cells (ab8956) can distinguish and quantitate the four major fractions of the cell cycle.

Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/100 - 1/200. ABC method.
IHC-P 1/100 - 1/200. ABC method.

ターゲット情報

  • 関連性
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 細胞内局在
    Nuclear
  • 別名
    • Bromodeoxyuridine antibody
    • BUdr antibody

画像

  • Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells with ab8955.

     

参考文献

This product has been referenced in:
  • Andrews WD  et al. Altered proliferative ability of neuronal progenitors in PlexinA1 mutant mice. J Comp Neurol 524:518-34 (2016). Read more (PubMed: 25975775) »
  • Birey F & Aguirre A Age-Dependent Netrin-1 Signaling Regulates NG2+ Glial Cell Spatial Homeostasis in Normal Adult Gray Matter. J Neurosci 35:6946-51 (2015). IHC-FrFl ; Mouse . Read more (PubMed: 25926469) »

See all 10 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Other
Permeabilization
Yes - 0.3% triton x-100
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3 · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Jacob Garza

Verified customer

投稿 Sep 14 2017

We unfortunately do not have any data confirming its cross reactivity with chlorodeoxyuridine. We however have customer feedback that say it does not bind to CldU or Idu but there is no tested data available. In case you are interested in buying and...

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Vielen Dank für Ihren wirklich sehr netten Anruf und für Ihr Interesse an unseren Produkten. Unseres Wissens nach wurde der Antikörper ab8955 bisher noch nicht in IHC-FrFl getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein s...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (BMSC)
Specification
BMSC
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Apr 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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