Anti-BrdU 抗体 [BU1/75 (ICR1)] (FITC) (ab74545)


  • 製品名
    Anti-BrdU antibody [BU1/75 (ICR1)] (FITC)
    BrdU 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [BU1/75 (ICR1)] to BrdU (FITC)
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • 特異性
    This antibody recognises BrdU incorporated into single stranded DNA, attached to a protein carrier or free BrdU. The antibody detects nucleated cells in S-phase which have had BrdU incorporated into their DNA. The antibody does not cross react with thymidine but does react weakly with chlorodeoxyuridine.
  • アプリケーション
    適用あり: Flow Cyt, ICC/IF, IHC-Fr, IHC-Pmore details
  • 種交差性
    Not applicable.
  • 免疫原

    The details for the immunogen of this antibody are not available.

  • 特記事項

    This product will be discontinued shortly. We recommend the following product as an alternative:
    Anti-BrdU antibody (Alexa Fluor® 488) [BU1/75 (ICR1)] (ab220074)



Our Abpromise guarantee covers the use of ab74545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/1 - 1/20.

Use 20ul of the suggested working dilution to label 106 cells in 100ul.
ab18446 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 21527773
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.


  • 関連性
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 細胞内局在
  • 別名
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody [BU1/75 (ICR1)] (FITC) 画像

  • Detection of BrdU in cells using ab74545 counterstained with propidium iodide.

Anti-BrdU antibody [BU1/75 (ICR1)] (FITC) (ab74545) 使用論文

This product has been referenced in:
  • Jia HY  et al. Asymmetric stem-cell division ensures sustained keratinocyte hyperproliferation in psoriatic skin lesions. Int J Mol Med 37:359-68 (2016). IHC ; Mouse . Read more (PubMed: 26707630) »
  • García-Sánchez O  et al. Interferon-? Reduces the Proliferation of Primed Human Renal Tubular Cells. Nephron Extra 4:1-7 (2014). Flow Cyt . Read more (PubMed: 24575118) »

See all 8 Publications for this product

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