製品の概要

  • 製品名Anti-BrdU antibody (Biotin)
    BrdU 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to BrdU (Biotin)
  • 標識Biotin
  • 特異性The antibody was tested using immunoprecipitation against 5-Methyl Cytosine (5-MeC) and bromo-deoxyuridine (BrdU) or control (no antigen) and assayed by A-405 spectrophotometry. At a concentration of 25 µg/mL, this product demonstrates 8 fold higher reactivity against BrdU than 5-MeC. This product titers out at 50 ng/mL. For best results, use the product at a concentration of 25 to 100 µg/mL. Nearly complete immunoprecipitation was obtained at a concentration of 100 to 500 µg/mL.
  • アプリケーション適用あり: IHC-P, IHC-Fr, IP, ICC/IF, ELISA, IHC-FoFrmore details
  • 免疫原

    Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution. Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10-3 N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail.
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution. dilute from 1/500 to 1/40,000 against 1mg/mL BrdU analyte.
IHC-FoFr 1/2000. 1/2000 (see Abreview).

ターゲット情報

  • 関連性The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 細胞内局在Nuclear
  • 別名
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody (Biotin) 画像

  • ab2284 at 1/2000 dilution staining mouse free floating brain slices by Immunohistochemistry (Formalin/PFA fixed sections). The mice were treated with 100mg/kg BrdU 2 hours before fixation. Free floating 40µm vibratome sections were obtained from paraformaldehyde fixed brains, these were incubated with the antibody for 24 hours. A streptavidin-HRP complex and DAB were used for detection. The image depicts the subventricular zone.

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  • ab2284 at 1/250 staining primary E12 mouse cortex cells by ICC/IF. The cells were paraformaldehyde fixed, blocked with serum and then incubated with the antibody for 24 hours. Streptavidin conjugated to Alexa-Fluor ®  488 was used as the secondary. The image shows BrdU staining with nuclei counterstained with DAPI.

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  • ab2284 staining BrdU in mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% H2O2 in methanol for 12 minutes; antigen retrieval was by heat mediation in 10mM citrate, pH6. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C.

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Anti-BrdU antibody (Biotin) (ab2284) 使用論文

This product has been referenced in:

See all 19 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skin)
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10%
Fixative Acetone
Username

Ahmar Aziz

Verified customer

投稿 Feb 10 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate, pH6
Sample Mouse Tissue sections (intestine)
Specification intestine
Blocking step (agent) for 12 minute(s) · Concentration: 1%
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 09 2014

Thank you very much for your call today and for letting us know about the trouble with this antibody.

As we discussed, I'm sending a vial of the rat monoclonal anti-BrdUab6326 on the order ***, which should arrive tomorrow.

I am hop...

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Thank you for your reply.

This antibody can be at -80C in aliquots for storage, and this may extend the life of the antibody. Either -20C or -80C will be fine.

Please let me know if you have any further questions, and I look forward...

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Thank you for your reply and forsending the additional information.

It does sound like the antibody was damaged during storage, so I am happy to send a replacement vial for you. This will be coming free of charge on the order ***, which shoul...

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Thank you for your inquiry.

You will denature the DNA first and then carry on with the IHC staining protocol.

http://www.abcam.com/index.html?pageconfig=resource&rid=11533
http://www.abcam.com/assets/popups/popup_datasheet_pr...

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Vielen Dank für Ihren wirklich sehr netten Anruf und für Ihr Interesse an unseren Produkten. Unseres Wissens nach wurde der Antikörper ab8955 bisher noch nicht in IHC-FrFl getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein s...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse tumour)
Specification Mouse tumour
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - 0.1% Triton X-100
Blocking step Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 22 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (brain,lateral ventricle)
Specification brain,lateral ventricle
Fixative Paraformaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Tris Buffered Saline- 0.3% Triton X
Permeabilization Yes - 4N HCl
Blocking step NDS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. stefano gaburro

Verified customer

投稿 Nov 01 2007

Thank you for your enquiry. I managed to find some references that you might be interested in. 1) Hiraga,et al. 2005. DNA polymerases alpha, delta, and epsilon localize and function together at replication forks in Saccharomyces cerevisiae. Gen...

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1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"