製品の概要

  • 製品名Anti-BrdU antibody
    BrdU 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to BrdU
  • アプリケーション適用あり: IHC-P, WB, ELISA, ICC/IFmore details
  • 免疫原

    BrdU-KLH in Freunds adjuvant.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 1 µg/ml.
ELISA Use a concentration of 1 - 0.1 µg/ml.
ICC/IF Use a concentration of 1 - 0.5 µg/ml.

ターゲット情報

  • 関連性The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • 細胞内局在Nuclear
  • 別名
    • Bromodeoxyuridine antibody
    • BUdr antibody

Anti-BrdU antibody 画像

  • ab92837, at 5.0 µg/ml, staining BrdU by Immunohistochemistry. Image shown was generated using formalin fixation.

Anti-BrdU antibody (ab92837) 使用論文

This product has been referenced in:
  • Itsara LS  et al. Oxidative stress is not a major contributor to somatic mitochondrial DNA mutations. PLoS Genet 10:e1003974 (2014). WB ; Drosophila melanogaster . Read more (PubMed: 24516391) »
  • Liu F  et al. Hydrogen sulfide improves wound healing via restoration of endothelial progenitor cell functions and activation of angiopoietin-1 in type 2 diabetes. Diabetes 63:1763-78 (2014). Read more (PubMed: 24487028) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (heart)
Permeabilization Yes - 0.3% triton x
Specification heart
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Feb 16 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (colon)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6.0
Permeabilization Yes - 0.01% Triton X-100
Specification colon
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Formaldehyde
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投稿 Sep 23 2015



1.) I can confirm that I only know of few disadvantages of chicken IgY and they all do not apply to IHC:

There are some disadvantages when using certain chicken derived antibodies in immunoassays. Chicken IgY does not fix mammalian ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"