製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BRD7
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chicken, Cow, Dog, Zebrafish, Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human BRD7.

    (Peptide available as ab73296.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: TE 671, SW480, SHSY-5Y, HeLa - Hydroxyurea Treated (48hr, 2µM) (data not shown)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab46553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).

ターゲット情報

  • 機能
    Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR (By similarity). Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase.
  • 配列類似性
    Contains 1 bromo domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 75 kDa bromodomain protein antibody
    • BP75 antibody
    • BRD 7 antibody
    • BRD7 antibody
    • BRD7_HUMAN antibody
    • Bromodomain containing 7 antibody
    • bromodomain containing protein 7 antibody
    • Bromodomain-containing protein 7 antibody
    • CELTIX 1 antibody
    • CELTIX1 antibody
    • NAG4 antibody
    • Protein CELTIX-1 antibody
    see all

画像

  • All lanes : Anti-BRD7 antibody (ab46553) at 1/250 dilution

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : SW480 whole cell lysate (ab3957)
    Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 150 kDa,16 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

プロトコール

参考文献

ab46553 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your inquiry.

These two antibodies (ab46553, ab56036) you mentioned are polyclonal antibodies. Polyclonal antibodies consist of a mixed population of IgGs each of which will recognize a different epitope. Generally, epitope map...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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