製品の概要

  • 製品名Anti-Brd4 antibody
    Brd4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Brd4
  • アプリケーション適用あり: IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1312 and 1362 of human (NP_490597.1).

  • ポジティブ・コントロール
    • Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Placenta, Prostate Carcinoma Mouse controls: Colon Carcinoma CT26, Renal Cell Carcinoma, Squamous Cell Carcinoma
  • 特記事項

    Concentration is optimized for IHC and not determined

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 1 µg/mg of lysate.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能Plays a role in a process governing chromosomal dynamics during mitosis.
  • 組織特異性Ubiquitously expressed.
  • 関連疾患Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.
  • 配列類似性Contains 2 bromo domains.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Brd4 antibody
    • BRD4-NUT FUSION antibody
    • BRD4-NUT fusion oncoprotein antibody
    • BRD4_HUMAN antibody
    • Bromodomain containing 4 antibody
    • bromodomain containing protein 4 antibody
    • Bromodomain-containing protein 4 antibody
    • CAP antibody
    • chromosome associated protein antibody
    • HUNK1 antibody
    • HUNKI antibody
    • MCAP antibody
    • Mitotic chromosome-associated protein antibody
    • Protein HUNK1 antibody
    see all

Anti-Brd4 antibody 画像

  • ab84776 Immunoprecipitating Brd4 in human HEK293 whole cell lysate. 1000µg of cell lysate was incubated with primary antibody (1 µg/mg lysate in 50mM TRIS) and matrix (Protein G) for 16 hours at 4°C. For western blotting ab131366 anti-mouse HRP (1/10000) was used to confirm successful immunoprecipation.

     

    See Abreview

  • Staining of Brd4 by Immunohistochemistry on a formalin fixed, paraffin embedded section of human colon carcinoma using a 1/500 dilution of ab84776. Detection: DAB staining
  • Staining of Brd4 by Immunohistochemistry on a formalin fixed, paraffin embedded section of mouse teratoma using a 1/100 dilution of ab84776. Detection: DAB staining

Anti-Brd4 antibody (ab84776) 使用論文

ab84776 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Total protein in input 1000 µg
Username

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投稿 May 07 2014

The concentration of the Brd4 antibody ab84776 is 0.1mg/ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"