製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Brd4
  • アプリケーション
    適用あり: IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 1312 and 1362 of human (NP_490597.1).

  • ポジティブ・コントロール
    • Human controls: Anaplastic Thyroid Carcinoma, Breast Carcinoma, Colon Carcinoma, Ovarian Carcinoma, Pancreatic Islet Cell Tumor, Placenta, Prostate Carcinoma Mouse controls: Colon Carcinoma CT26, Renal Cell Carcinoma, Squamous Cell Carcinoma
  • 特記事項

    Concentration is optimized for IHC and not determined

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 1 µg/mg of lysate.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能
    Plays a role in a process governing chromosomal dynamics during mitosis.
  • 組織特異性
    Ubiquitously expressed.
  • 関連疾患
    Note=A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUT which produces a BRD4-NUT fusion protein.
  • 配列類似性
    Contains 2 bromo domains.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Brd4 antibody
    • BRD4-NUT FUSION antibody
    • BRD4-NUT fusion oncoprotein antibody
    • BRD4_HUMAN antibody
    • Bromodomain containing 4 antibody
    • bromodomain containing protein 4 antibody
    • Bromodomain-containing protein 4 antibody
    • CAP antibody
    • chromosome associated protein antibody
    • HUNK1 antibody
    • HUNKI antibody
    • MCAP antibody
    • Mitotic chromosome-associated protein antibody
    • Protein HUNK1 antibody
    see all

Anti-Brd4 antibody 画像

  • Staining of Brd4 by Immunohistochemistry on a formalin fixed, paraffin embedded section of human colon carcinoma using a 1/500 dilution of ab84776. Detection: DAB staining
  • Staining of Brd4 by Immunohistochemistry on a formalin fixed, paraffin embedded section of mouse teratoma using a 1/100 dilution of ab84776. Detection: DAB staining
  • ab84776 Immunoprecipitating Brd4 in human HEK293 whole cell lysate. 1000µg of cell lysate was incubated with primary antibody (1 µg/mg lysate in 50mM TRIS) and matrix (Protein G) for 16 hours at 4°C. For western blotting ab131366 anti-mouse HRP (1/10000) was used to confirm successful immunoprecipation.

     

    See Abreview

Anti-Brd4 antibody (ab84776) 使用論文

This product has been referenced in:
  • Yoshida H  et al. Brd4 bridges the transcriptional regulators, Aire and P-TEFb, to promote elongation of peripheral-tissue antigen transcripts in thymic stromal cells. Proc Natl Acad Sci U S A 112:E4448-57 (2015). WB . Read more (PubMed: 26216992) »

See 1 Publication for this product

Product Wall

Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Total protein in input
1000 µg
Username

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Verified customer

投稿 May 07 2014

The concentration of the Brd4 antibody ab84776 is 0.1mg/ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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