製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to BRD2
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide (Human) - which represented a portion of human Bromodomain Containing 2 (BRD 2) between the putative nuclear localization signal and the ET domain.

  • ポジティブ・コントロール
    • HeLa cell extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/40000. Predicted molecular weight: 110 kDa.
ICC/IF Use at an assay dependent concentration. PubMed: 22215678

ターゲット情報

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  • Predicted band size : 110 kDa


    Sample: Hela cell total RIPA extract (10µg) Antibody: Affinity purified Goat anti-BRD 2 (ab3718) (diluted 1 / 20,000) CIP: Calf Intestinal Phosphatase Detection: ECL exposed for 1 minute.

参考文献

This product has been referenced in:
  • Sun Y  et al. BET protein inhibition mitigates acute myocardial infarction damage in rats via the TLR4/TRAF6/NF-?B pathway. Exp Ther Med 10:2319-2324 (2015). WB . Read more (PubMed: 26668635) »
  • Dhar S  et al. Insights into role of bromodomain, testis-specific (Brdt) in acetylated histone H4-dependent chromatin remodeling in mammalian spermiogenesis. J Biol Chem 287:6387-405 (2012). ICC/IF ; Rat . Read more (PubMed: 22215678) »

See all 5 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (embryonic fibroblasts)
Loading amount
15 µg
Specification
embryonic fibroblasts
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 22 2013

Thank you for contacting us.

Yes, ab3718 can recognise both the phosphorylated and unphosphorylated BRD2.

For ab113117, we recommend to use 1ug/ml (1:1000) in WB.

I hope this information is helpful to you. Please do not h...

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Thank you for contacting us.

The target protein got a number of phosphorserine and phosphothreonine residues which can increase the molecular weight of the protein.

I hope this information is helpful to you. Please do not hesitate ...

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Thank you for your enquiry. I have requested that the lab performs an alignment of the immunogen sequences with the rat homologs. Details of the degree of similarity is below: BRD2(rat) - GeneID: 294276 (NP_997660) The peptide immunogen w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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