Anti-BRCC45/BRE 抗体 [EPR11858] (ab177960)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11858] to BRCC45/BRE
- Suitable for: WB, ICC/IF, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-BRCC45/BRE antibody [EPR11858]
BRCC45/BRE 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11858] to BRCC45/BRE -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide within Human BRCC45/BRE aa 1-100. The exact sequence is proprietary.
Database link: Q9NXR7 -
ポジティブ・コントロール
- WB: HeLa, SH-SY5Y, Daudi and Raji cell lysates; Fetal brain and human placenta tissue lysates. IHC-P: Human lung adenocarcinoma and pancreas tissue. ICC/IF: MCF-7 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR11858 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177960の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
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ICC/IF |
1/50 - 1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
ICC/IF
1/50 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect. -
組織特異性
Expressed in all cell lines examined. Highly expressed in placenta. -
配列類似性
Belongs to the BRE family. -
ドメイン
Contains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin. -
細胞内局在
Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks. - Information by UniProt
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参照データベース
- Entrez Gene: 9577 Human
- Entrez Gene: 107976 Mouse
- Entrez Gene: 362704 Rat
- Omim: 610497 Human
- SwissProt: Q9NXR7 Human
- SwissProt: Q8K3W0 Mouse
- SwissProt: Q6P7Q1 Rat
- Unigene: 258314 Human
see all -
別名
- 6030405P19Rik antibody
- AI429776 antibody
- B830038C02Rik antibody
see all
画像
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All lanes : Anti-BRCC45/BRE antibody [EPR11858] (ab177960) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BRCC45/BRE knockout HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 44 kDaLanes 1-4: Merged signal (red and green). Green - ab177960 observed at 44 kDa. Red - loading control ab7291 observed at 50 kDa.
ab177960 Recombinant Anti-BRE antibody [EPR11858] was shown to specifically react with BRE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264928 (knockout cell lysate ab257861) was used. Wild-type and BRE knockout samples were subjected to SDS-PAGE. ab177960 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of Human pancreas tissue labeling BRCC45/BRE using ab177960 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescence analysis of MCF-7 cells labeling BRCC45/BRE using ab177960 at 1/50 dilution.
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All lanes : Anti-BRCC45/BRE antibody [EPR11858] (ab177960) at 1/1000 dilution
Lane 1 : fetal brain lysate
Lane 2 : Human placenta lysate
Lane 3 : HeLa cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 44 kDa -
Immunohistochemical analysis of Human lung adenocarcinoma tissue labeling BRCC45/BRE using ab177960 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab177960 は 2 報の論文で使用されています。
- Jin F et al. BRE Promotes Esophageal Squamous Cell Carcinoma Growth by Activating AKT Signaling. Front Oncol 10:1407 (2020). PubMed: 32850455
- Ramos H et al. Beneficial Effects of Glucagon-Like Peptide-1 (GLP-1) in Diabetes-Induced Retinal Abnormalities: Involvement of Oxidative Stress. Antioxidants (Basel) 9:N/A (2020). PubMed: 32927585