製品の概要

  • 製品名Anti-BRCC45 antibody
    BRCC45 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BRCC45
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 51-239 of Human BRCC45

  • ポジティブ・コントロール
    • A431, H1299, HeLa, HepG2, Molt-4 and Raji cells. Cal27 xenograft.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.
  • 組織特異性Expressed in all cell lines examined. Highly expressed in placenta.
  • 配列類似性Belongs to the BRE family.
  • ドメインContains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin.
  • 細胞内局在Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 6030405P19Rik antibody
    • AI429776 antibody
    • B830038C02Rik antibody
    • Brain and reproductive organ expressed (TNFRSF1A modulator) antibody
    • brain and reproductive organ expressed protein antibody
    • Brain and reproductive organ-expressed protein antibody
    • BRCA1 A complex subunit BRE antibody
    • BRCA1-A complex subunit BRE antibody
    • BRCA1/BRCA2 containing complex subunit 4 antibody
    • BRCA1/BRCA2 containing complex subunit 45 antibody
    • BRCA1/BRCA2-containing complex subunit 45 antibody
    • BRCC4 antibody
    • BRCC45 antibody
    • bre antibody
    • BRE_HUMAN antibody
    see all

Anti-BRCC45 antibody 画像

  • Anti-BRCC45 antibody (ab95985) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 47 kDa
  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelled with ab95985 at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labelled with ab95985 at 1/100 dilution.

Anti-BRCC45 antibody (ab95985) 使用論文

ab95985 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (293 cells)
Specification 293 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jul 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"