製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BRCC45
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 9 and 338 of Human BRCC45 (NP_004890).

  • ポジティブ・コントロール
    • HepG2, HeLa and NIH-3T3 whole cell lysates; 293T, A431, H1299, HeLaS3, MOLT4 and Raji cell lysates ICC/IF: HeLa cells

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it acts as an adapter that bridges the interaction between BABAM1/NBA1 and the rest of the complex, thereby being required for the complex integrity and modulating the E3 ubiquitin ligase activity of the BRCA1-BARD1 heterodimer. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. May play a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. May also act as a death receptor-associated anti-apoptotic protein, which inhibits the mitochondrial apoptotic pathway. May regulate TNF-alpha signaling through its interactions with TNFRSF1A; however these effects may be indirect.
  • 組織特異性
    Expressed in all cell lines examined. Highly expressed in placenta.
  • 配列類似性
    Belongs to the BRE family.
  • ドメイン
    Contains 2 ubiquitin-conjugating enzyme family-like (UEV-like) regions. These regions lack the critical Cys residues required for ubiquitination but retain the ability to bind ubiquitin.
  • 細胞内局在
    Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 6030405P19Rik antibody
    • AI429776 antibody
    • B830038C02Rik antibody
    • Brain and reproductive organ expressed (TNFRSF1A modulator) antibody
    • brain and reproductive organ expressed protein antibody
    • Brain and reproductive organ-expressed protein antibody
    • BRCA1 A complex subunit BRE antibody
    • BRCA1-A complex subunit BRE antibody
    • BRCA1/BRCA2 containing complex subunit 4 antibody
    • BRCA1/BRCA2 containing complex subunit 45 antibody
    • BRCA1/BRCA2-containing complex subunit 45 antibody
    • BRCC4 antibody
    • BRCC45 antibody
    • bre antibody
    • BRE_HUMAN antibody
    see all

画像

  • All lanes : Anti-BRCC45 antibody (ab95984) at 1/500 dilution

    Lane 1 : HeLa S3 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 47 kDa
  • Immunofluorescence analysis of BRCC45 in paraformaldehyde fixed HeLa, using ab95984 at 1/200 dilution. Lower image merged with DNA probe.
  • Anti-BRCC45 antibody (ab95984) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg

    Predicted band size : 47 kDa

参考文献

ab95984 has not yet been referenced specifically in any publications.

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