製品の概要

  • 製品名Anti-BRCC36 antibody
    BRCC36 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BRCC36
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Raised against an 18 amino acid peptide from near the amino terminus of human BRCC36.

  • ポジティブ・コントロール
    • MCF7 nuclear lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab62075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 36 kDa.
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.

ターゲット情報

  • 機能Metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:19214193, PubMed:20656690, PubMed:24075985, PubMed:26344097). Does not have activity toward 'Lys-48'-linked polyubiquitin chains. Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) (PubMed:20656690). Catalytic subunit of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:20656690, PubMed:24075985, PubMed:26344097, PubMed:26195665). Mediates the specific 'Lys-63'-specific deubiquitination associated with the COP9 signalosome complex (CSN), via the interaction of the BRISC complex with the CSN complex (PubMed:19214193). The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985, PubMed:26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).
  • 組織特異性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Aberrantly expressed in the vast majority of breast tumors.
  • 関連疾患A chromosomal aberration involving BRCC3 is a cause of pro-lymphocytic T-cell leukemia (T-PLL). Translocation t(X;14)(q28;q11) with TCRA.
  • 配列類似性Belongs to the peptidase M67A family. BRCC36 subfamily.
    Contains 1 MPN (JAB/Mov34) domain.
  • 細胞内局在Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Localizes at sites of DNA damage at double-strand breaks (DSBs) (PubMed:20656690, PubMed:26344097). Interaction with FAM175B/ABRO1 retains BRCC3 in the cytoplasm (PubMed:20656690).
  • Information by UniProt
  • 参照データベース
  • 別名
    • BRCA1-A complex subunit BRCC36 antibody
    • BRCA1/BRCA2 containing complex subunit 3 antibody
    • BRCA1/BRCA2 containing complex subunit 36 antibody
    • BRCA1/BRCA2-containing complex subunit 3 antibody
    • BRCA1/BRCA2-containing complex subunit 36 antibody
    • Brcc3 antibody
    • BRCC3_HUMAN antibody
    • BRCC36 antibody
    • BRISC complex subunit BRCC36 antibody
    • C6.1A antibody
    • Chromosome X open reading frame 53 antibody
    • CXorf53 antibody
    • Lys-63-specific deubiquitinase BRCC36 antibody
    • OTTHUMP00000061450 antibody
    see all

Anti-BRCC36 antibody 画像

  • Lane 1 : Anti-BRCC36 antibody (ab62075) at 0.5 µg/ml
    Lane 2 : Anti-BRCC36 antibody (ab62075) at 1 µg/ml
    Lane 3 : Anti-BRCC36 antibody (ab62075) at 2 µg/ml

    Lane 1 : MCF7 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    anti-rabbit IgG

    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • ab62075 at 2.5 µg/ml staining BRCC36 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • Immunofluorescence of BRCC36 in Human Brain cells using ab62075 at 20 ug/ml.

Anti-BRCC36 antibody (ab62075) 使用論文

ab62075 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab62075.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"