製品の概要

  • 製品名Anti-BRAP antibody
    BRAP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to BRAP
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 BRAP.

    (Peptide available as ab88155.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in MCF7 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
ICC/IF 1/200. Use with paraformaldehyde fixed cells.

ターゲット情報

  • 関連性BRCA1 Associated Protein, BRAP2 (Impedes mitogenic signal propogation; IMP) is mainly cytoplasmic and binds to the NLS motifs of BRCA1 with similar specificity to that of importin alpha. BRAP2 may function as a cytoplasmic retention protein and play a role in regulating transport of nuclear proteins. More recently, the protein has been shown to impede signal transmission from Raf to MEK.
  • 細胞内局在Cytoplasmic
  • 参照データベース
  • 別名
    • 3010002G07Rik antibody
    • BRAP2 antibody
    • BRCA1 associated protein antibody
    • EC 6.3.2. antibody
    • Galectin 2 binding protein antibody
    • IMP antibody
    • Impedes mitogenic signal propagation antibody
    • Renal carcinoma antigen NY REN 63 antibody
    • RING finger protein 52 antibody
    • RNF52 antibody
    • zgc:92894 antibody
    see all

Anti-BRAP antibody 画像

  • Anti-BRAP antibody (ab77721) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 67 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 12 kDa,34 kDa. We are unsure as to the identity of these extra bands.
  • ab77721 (1/200) staining BRAP in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

Anti-BRAP antibody (ab77721) 使用論文

ab77721 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

投稿 Aug 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"