製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BRAP
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Chicken
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 BRAP.

    (Peptide available as ab88155.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in MCF7 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
ICC/IF 1/200. Use with paraformaldehyde fixed cells.

ターゲット情報

  • 関連性
    BRCA1 Associated Protein, BRAP2 (Impedes mitogenic signal propogation; IMP) is mainly cytoplasmic and binds to the NLS motifs of BRCA1 with similar specificity to that of importin alpha. BRAP2 may function as a cytoplasmic retention protein and play a role in regulating transport of nuclear proteins. More recently, the protein has been shown to impede signal transmission from Raf to MEK.
  • 細胞内局在
    Cytoplasmic
  • 参照データベース
  • 別名
    • 3010002G07Rik antibody
    • BRAP2 antibody
    • BRCA1 associated protein antibody
    • EC 6.3.2. antibody
    • Galectin 2 binding protein antibody
    • IMP antibody
    • Impedes mitogenic signal propagation antibody
    • Renal carcinoma antigen NY REN 63 antibody
    • RING finger protein 52 antibody
    • RNF52 antibody
    • zgc:92894 antibody
    see all

画像

  • Anti-BRAP antibody (ab77721) at 1 µg/ml + MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 67 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 12 kDa, 34 kDa. We are unsure as to the identity of these extra bands.



    BRAP contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ab77721 (1/200) staining BRAP in assynchronous HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

参考文献

ab77721 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
10 µg
Specification
heart
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Mar 05 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton X100
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Aug 03 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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