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Abcam's Branched Chain Amino Acid Assay Kit provides a simple convenient means of measuring the BCAA's in a variety of biological samples. The kit utilizes an enzyme assay in which BCAA is oxidatively deaminated, producing NADH which reduces the probe, generating a colored product (?max = 450 nm). Abcam's Branched Chain Amino Acid Assay Kit measures BCAA's in the range of 0 to 10 nmol per sample with a detection limit of ~0.2 nmol (~10 µM BCAA in sample). BCAA's are present in serum ~ 0.1-0.4 mM each (~0.125-1.5 mM combined).
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The branched-chain amino acids or BCAA's, refer to the amino acids with non-linear aliphatic side-chains, namely leucine, isoleucine and valine. These three essential amino acids make up approximately 1/3 of skeletal muscle in the human body. BCAA's are currently used clinically to aid in the recovery of burn victims, as well as for strength supplementation for athletes. BCAA's, primarily Leu, can stimulate insulin secretion. The BCAA's have also been implicated in a wide range of other physiological effects.
|BCAA Assay Buffer||WM||1 x 25ml|
|BCAA Enzyme Mix (lyophilized)||Green||1 vial|
|Leu Standard (1 µmol)||Yellow||1 x 100µl|
|WST Substrate Mix (lyophilized)||Red||1 vial|
Our Abpromise guarantee covers the use of ab83374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
BRCAA levels measured in mouse tissue lysates (mg of extracted protein; background signal subtracted, mean of duplicates; +/- SD).
BRCAA levels measured in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
BRCAA levels measured in human biological fluids (background signal subtracted, mean of duplicates; +/- SD).