製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to BPTF / FALZ
  • アプリケーション
    適用あり: ICC/IF, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Orangutan
  • 免疫原

    Synthetic peptide from a region between residue 1350 and 1400 of human BPTF/ FALZ, NP_872579.2

  • ポジティブ・コントロール
    • Whole cell lysate from Hela cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/2000 - 1/10000. Detects a band of approximately 324 kDa (predicted molecular weight: 324 kDa).

Several other bands.

IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Histone-binding component of NURF (nucleosome-remodeling factor), a complex which catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. Specifically recognizes H3 tails trimethylated on 'Lys-4' (H3K4me3), which mark transcription start sites of virtually all active genes. May also regulate transcription through direct binding to DNA or transcription factors.
  • 組織特異性
    Ubiquitously expressed, with highest levels in testis. Present in kidney, liver and brain. In the brain, highest levels are found in motor cortex (at protein level).
  • 配列類似性
    Belongs to the PBTF family.
    Contains 1 bromo domain.
    Contains 1 DDT domain.
    Contains 2 PHD-type zinc fingers.
  • 発生段階
    Abundantly expressed in the fetal brain. Present throughout the gray and white matter of the developing spinal cord at 18-22 gestational weeks. Expressed at low levels in adult brain and spinal cord and reexpressed in neurodegenerative diseases (at protein level).
  • ドメイン
    The second PHD-type zinc finger mediates binding to histone H3K4Me3. Has specificity for trimethyllysine; introducing a mutation in the Tyr-2876 residue can induce binding to dimethyllysine.
  • 翻訳後修飾
    Phosphorylation enhances DNA-binding.
    Highly susceptible to proteolysis.
  • 細胞内局在
    Cytoplasm. Nucleus. In brains of Alzheimer disease patients, present in a subset of amyloid-containing plaques.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BPTF antibody
    • BPTF_HUMAN antibody
    • Bromodomain and PHD finger containing transcription factor antibody
    • Bromodomain and PHD finger-containing transcription factor antibody
    • Bromodomain PHD finger transcription factor antibody
    • FAC 1 antibody
    • fac1 antibody
    • FALZ antibody
    • Fetal Alz 50 clone 1 protein antibody
    • Fetal Alz 50 reactive clone 1 antibody
    • Fetal Alz-50 clone 1 protein antibody
    • Fetal Alzheimer antigen antibody
    • Nucleosome remodeling factor antibody
    • Nucleosome remodeling factor subunit BPTF antibody
    • Nucleosome remodeling factor, large subunit antibody
    • Nucleosome-remodeling factor subunit BPTF antibody
    • NURF 301 antibody
    • NURF301 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BPTF / FALZ with ab72036 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-BPTF / FALZ antibody (ab72036) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells. at 50 µg
    Lane 2 : Whole cell lysate from Hela cells. at 15 µg
    Lane 3 : Whole cell lysate from Hela cells. at 5 µg


    Predicted band size : 324 kDa
    Observed band size : 324 kDa
    Various other bands are detected from 117 to 460 kDa.
  • Immunoprecipitation/ Western Blot of FALZ / BPTF.
    Lane 1: ab72036 at 3µg/mg whole cell lysate for IP, and 0.1 µg/ml for subsequent WB.
    Lane 2: Control IgG.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Detection: Chemiluminescence with an exposure time of 1 second.
  • ICC/IF image of ab72036 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72036, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Richart L  et al. BPTF is required for c-MYC transcriptional activity and in vivo tumorigenesis. Nat Commun 7:10153 (2016). WB ; Human . Read more (PubMed: 26729287) »
  • Mulder KW  et al. Diverse epigenetic strategies interact to control epidermal differentiation. Nat Cell Biol 14:753-63 (2012). ChIP . Read more (PubMed: 22729083) »

See all 2 Publications for this product

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